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| NucleoSpin® Plant II Midi / Maxi |
Genomic DNA from plant and fungi
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Features
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| For rapid medium and large scale preparation of genomic DNA from plant cells and fungi |
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• Two alternative lysis buffers included for optimal processing of various samples and highest flexibility:
Lysis Buffer PL1, based on CTAB lysis method
Lysis Buffer PL2, based on SDS lysis method
• NucleoSpin® Filters L / XL included for clarification of lysate
• Improved buffer compositions → higher yield and purity
• RNase A included
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NucleoSpin® Plant II Midi
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NucleoSpin® Plant II Maxi
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| Technology |
Silica-membrane technology
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| Format |
Midi spin columns
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Maxi spin columns
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| Sample material |
<400 mg plant tissue (wet weight)
<80 mg plant tissue (dry weight)
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<1500 mg plant tissue (wet weight)
<300 mg plant tissue (dry weight)
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| Lysate clarification |
NucleoSpin® Filters L
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NucleoSpin® Filters XL
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| Fragment size |
50 bp–approx. 50 kbp
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50 bp–approx. 50 kbp
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| Typical yield |
10–100 µg
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50–300 µg
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| A260/A280 |
1.8–1.9
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1.8–1.9
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| Elution volume |
200–400 µL
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1000–3000 µL
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| Preparation time |
90 min/prep
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90 min/prep
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| Binding capacity |
200 µg
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500 µg
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Applications
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• DNA from plant cells and tissue
• DNA from fungi
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| • Typical downstream applications: PCR, Southern blotting, enzymatic reactions |
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoSpin® Plant II Midi |
20
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NucleoSpin® Plant II Midi Columns with Collection Tubes, NucleoSpin® Filters L, Collection Tubes (15 mL), buffers, RNase A
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740771.20 |
| NucleoSpin® Plant II Maxi |
10
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NucleoSpin® Plant II Maxi Columns with Collection Tubes, NucleoSpin® Filters XL, Collection Tubes (50 mL), buffers, RNase A
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740772.10 |
For ordering click here
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