NucleoBond AXG® 20	NucleoBond AXG® 100	NucleoBond AXG® 500
Technology	Anion-exchange chromatography
Format	Mini gravity flow columns	Midi gravity flow columns	Maxi gravity flow columns
Sample material	2–5 mL bacterial culture 3–5 mL yeast culture* < 20 mg tissue	15–20 mL bacterial culture 10–20 mL yeast culture* < 100 mg tissue	60–80 mL bacterial culture 100–500 mL yeast culture* < 400 mg tissue
Fragment size	500 bp–300 kbp	500 bp–300 kbp	500 bp–300 kbp
Typical yield	20 µg	100 µg	500 µg
A260/A280	1.80–1.95	1.80–1.95	1.80–1.95
Preparation time	4–5 h	4–5 h	4–5 h
Binding capacity	20 µg	100 µg	500 µg
Applications**
• Genomic DNA from bacteria, yeast*, tissue
*   For isolation of genomic DNA from yeast additional buffers are required, see user manual ** Kits to be used for research purposes only

Ordering information

Product	Preps	Specification	REF
NucleoBond® AXG columns without buffers
NucleoBond® AXG 20	20	NucleoBond® AXG 20 Columns	740544
NucleoBond® AXG 100	20	NucleoBond® AXG 100 Columns	740545
NucleoBond® AXG 500	10	NucleoBond® AXG 500 Columns	740546
NucleoBond® buffer sets
NucleoBond® Buffer Set III for isolation of genomic DNA from bacteria and yeast*		for 10 NucleoBond® AXG 500 Columns or 20 NucleoBond® AXG 100 Columns or 100 NucleoBond® AXG 20 Columns	740603
NucleoBond® Buffer Set IV for isolation of genomic DNA from tissue		for 10 NucleoBond® AXG 500 Columns or 20 NucleoBond® AXG 100 Columns or 100 NucleoBond® AXG 20 Columns	740604
Product accessories	Pack of	Specification	REF
NucleoBond® Small Rack	1	for use with NucleoBond® AXG 20 Columns	740562
NucleoBond® Large Rack	1	for use with NucleoBond® AXG 100 and 500 Columns	740563

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