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User Manual
NucleoBond AXG

  
     

NucleoBond® AXG

Genomic DNA from tissue and cells

Features 
The anion-exchanger columns for purification of up to 500 µg genomic DNA

• Ultra-pure genomic DNA from from tissue, bacteria, or yeast
• Combine NucleoBond AXG columns and the corresponding buffer set for highest flexibility:

• NucleoBond AXG columns + NucleoBond Buffer Set III ® genomic DNA from bacteria and yeast*
• NucleoBond AXG columns + NucleoBond Buffer Set IV ® genomic DNA from tissue

NucleoBond
AXG 20

NucleoBond
AXG 100

NucleoBond
AXG 500

Technology

Anion-exchange chromatography

Format

Mini
Gravity flow columns

Midi
Gravity flow columns

Maxi
Gravity flow columns

Sample material

2-5 ml bacterial culture
3-5 ml yeast culture*
< 20 mg tissue

15-20 ml bacterial culture
10-20 ml yeast culture*
< 100 mg tissue

60-80 ml bacterial culture
100-500 ml yeast culture*
< 400 mg tissue

Fragment size

500 bp - 300 kbp

500 bp - 300 kbp

500 bp - 300 kbp

Typical yield

20 µg

100 µg

500 µg

A260/280

1.80-1.95

1.80-1.95

1.80-1.95

Preparation time

 4-5 h

 4-5 h

 4-5 h

Binding capacity

20 µg

100 µg

500 µg

Applications**
• Genomic DNA from bacteria, yeast*, tissue
*   For isolation of genomic DNA from yeast additional buffers are required, see user manual
** Kits to be used for research purposes only

  
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Ordering information

Product

Preps

Specification Reference
NucleoBond AXG columns without buffers
NucleoBond AXG 20

20

NucleoBond AXG 20 Columns 740544
NucleoBond AXG 100

20

NucleoBond AXG 100 Columns 740545
NucleoBond AXG 500

10

NucleoBond AXG 500 Columns 740546
NucleoBond buffer sets
NucleoBond buffer set III
for isolation of genomic DNA from
bacteria and yeast*

 

for 10 NucleoBond AXG 500 Columns or
20 NucleoBond AXG 100 Columns or
100 NucleoBond AXG 20 Columns
740603
NucleoBond buffer set IV
for isolation of genomic DNA from
tissue

 

for 10 NucleoBond AXG 500 Columns or
20 NucleoBond AXG 100 Columns or
100 NucleoBond AXG 20 Columns
740604
Product accessories

Pack of

Specification Reference
NucleoBond Small Rack

1

for use with NucleoBond AXG 20 Columns 740562
NucleoBond Large Rack

1

for use with NucleoBond AXG 100 and 500 Columns 740563

For ordering click here

  
     
  
     
  
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