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NucleoMag® Blood 200 µL / 3 mL

DNA from blood and biological fluids


Features 

Magnetic-bead based isolation of genomic DNA from whole blood

• One-tube procedure minimizes risk of cross-contamination
• Small elution volumes:  50 µL (NucleoMag® Blood 200 µL), 1 mL (NucleoMag® Blood 3 mL)
• Complete processing possible at room temperature
• Easily adapted to automated use
• Consistently high yields

  

NucleoMag® Blood 200 µL

NucleoMag® Blood 3 mL

Technology

Magnetic-bead technology

Format

Highly reactive superparamagnetic beads

Processing

Manual or automated

Automated*

Sample material
200 µL whole blood
(fresh or frozen, EDTA or citrate treated)
3 mL whole blood
(fresh or frozen, EDTA or citrate treated)
Fragment size 20–approx. 50 kbp 20–approx. 50 kbp
Typical yield 2–8 µg 100–130 µg
A260/A280 1.6–1.9 1.6–1.9
Elution volume ≥ 50 µL ≥ 1 mL
Preparation time 45 min/ 96 preps* 60 min/24 preps*
Binding capacity Approx. 0.4 µg/µL Approx. 0.4 µg/µL


Applications**

• Manual or automated isolation of genomic DNA from whole blood
• Typical downstream applications: PCR, real-time PCR, Southern blotting, any kind of enzymatic reaction


* Established on KingFisher® Flex
** Kits to be used for research purposes only!


 

  
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Ordering information

Product

Preps

Specification REF
NucleoMag® Blood 200 µL

1 x 96

NucleoMag® B-Beads, buffers, Proteinase K 744501.1

4 x 96

as above 744501.4
NucleoMag® Blood 3 mL

1 x 96

NucleoMag® B-Beads, buffers, Proteinase K 744502.1
Material to be supplied by the user
Separation Plate, e.g. Square-well Block

4

740481

24

740481.24
Elution plate, e.g. Elution Plate U-bottom

24

740486.24

 

For use of NucleoMag® Blood 200 µL only on KingFisher® 96/Flex

 

KingFisher® 96 Accessory Kit B

1 set

Square-well Blocks, Deep-well Tip Combs and Elution Plates for 4 x 96 preparations 744951
Product accessories

Pack of

Specification REF
NucleoMag® SEP

1

magnetic separator 744900

For ordering click here

  
     
  
   
  
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