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NucleoMag® Blood 200 µL / 3 mL
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DNA from blood and biological fluids
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| Features |
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Magnetic-bead based isolation of genomic DNA from whole blood
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• One-tube procedure minimizes risk of cross-contamination
• Small elution volumes: ≥ 50 µL (NucleoMag® Blood 200 µL), ≥ 1 mL (NucleoMag® Blood 3 mL)
• Complete processing possible at room temperature
• Easily adapted to automated use
• Consistently high yields
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NucleoMag® Blood 200 µL
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NucleoMag® Blood 3 mL
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| Technology |
Magnetic-bead technology
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| Format |
Highly reactive superparamagnetic beads
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| Processing |
Manual or automated
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Automated*
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Sample material
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200 µL whole blood
(fresh or frozen, EDTA or citrate treated) |
3 mL whole blood
(fresh or frozen, EDTA or citrate treated) |
| Fragment size |
20–approx. 50 kbp |
20–approx. 50 kbp |
| Typical yield |
2–8 µg |
100–130 µg |
| A260/A280 |
1.6–1.9 |
1.6–1.9 |
| Elution volume |
≥ 50 µL |
≥ 1 mL |
| Preparation time |
45 min/ 96 preps* |
60 min/24 preps* |
| Binding capacity |
Approx. 0.4 µg/µL |
Approx. 0.4 µg/µL |
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| Applications** |
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• Manual or automated isolation of genomic DNA from whole blood
• Typical downstream applications: PCR, real-time PCR, Southern blotting, any kind of enzymatic reaction
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* Established on KingFisher® Flex
** Kits to be used for research purposes only!
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoMag® Blood 200 µL |
1 x 96
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NucleoMag® B-Beads, buffers, Proteinase K |
744501.1 |
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4 x 96
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as above |
744501.4 |
| NucleoMag® Blood 3 mL |
1 x 96
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NucleoMag® B-Beads, buffers, Proteinase K |
744502.1 |
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| Material to be supplied by the user |
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| Separation Plate, e.g. Square-well Block |
4
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740481 |
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24
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740481.24 |
| Elution plate, e.g. Elution Plate U-bottom |
24
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740486.24 |
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| For use of NucleoMag® Blood 200 µL only on KingFisher® 96/Flex |
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| KingFisher® 96 Accessory Kit B |
1 set
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Square-well Blocks, Deep-well Tip Combs and Elution Plates for 4 x 96 preparations |
744951 |
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| Product accessories |
Pack of
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Specification |
REF |
| NucleoMag® SEP |
1
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magnetic separator |
744900 |
For ordering click here
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