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NucleoSpin®
Blood L / XL

  
     

NucleoSpin® Blood L / XL

Genomic DNA from blood and biological fluids


Features 

For rapid medium scale purification of DNA from blood

• Ready-to-use DNA in less than 60 min
• Complete removal of PCR inhibitors
• Suitable for whole blood, treated with citrate, EDTA, heparin, CPDA
• Consistently high DNA yields



NucleoSpin® Blood L

NucleoSpin® Blood XL

Technology

Silica-membrane technology

Format

Midi spin columns

Maxi spin columns

Sample material

0.2–2 mL whole blood
2 x 107 cultured cells

2–10 mL whole blood
108 cultured cells

Fragment size

200 bp–approx. 50 kbp

200 bp–approx. 50 kbp

Typical yield

40–60 µg

200–300 µg

A260/A280

1.6–1.9

1.6–1.9

Typical concentration

200–500 ng/µL

200–500 ng/µL

Elution volume

120–200 µL

600–1000 µL

Preparation time

~60 min/prep

~60 min/prep

Binding capacity

250 µg

700 µg



Applications*


• DNA from whole blood (human or animal blood, fresh or frozen)
• DNA from whole blood treated with citrate, EDTA, heparin, CPDA
• DNA from serum, plasma, buffy coat, platelets, body fluids (e.g., amniotic fluid)
• DNA from cultured cells

• Typical downstream applications: PCR, Southern blotting, enzymatic reactions

* Kits to be used for research purposes only

 

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® Blood L

20

NucleoSpin® Blood L Columns with Collection Tubes, Collection Tubes (15 mL), buffers, Proteinase K 740954.20
NucleoSpin® Blood XL

10

NucleoSpin® Blood XL Columns with Collection Tubes, Collection Tubes (50 mL), buffers, Proteinase K 740950.10

50

as above 740950.50

For ordering click here

  
     
  
   
  
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