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| NucleoBond® CB |
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DNA from blood and biological fluids
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| Features |
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The anion exchanger for purification of up to 500 µg genomic DNA from whole blood
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• Ultra-pure genomic DNA from different samples
• Kits for 100–500 μg genomic DNA
• Sample size up to 20 mL whole blood
• High molecular weight DNA up to 300 kbp
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NucleoBond® CB 20
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NucleoBond® CB 100
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NucleoBond® CB 500
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| Technology |
Anion-exchange chromatography
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| Format |
Mini gravity-flow columns
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Midi gravity-flow columns
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Maxi gravity-flow columns
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| Sample material |
0.1–1 mL whole blood
< 50 µL buffy coat
5 x 106 cells
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2–5 mL whole blood
< 250 µL buffy coat
2 x 107 cells
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5–20 mL whole blood
< 1 mL buffy coat
108 cells
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| Fragment size |
500 bp–300 kbp
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500 bp–300 kbp
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500 bp–300 kbp
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Typical yield
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20 µg
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100 µg
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500 µg
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| A260/A280 |
1.80–1.95
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1.80–1.95
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1.80–1.95
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| Preparation time |
4–5 h
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4–5 h
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4–5 h
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| Binding capacity |
20 µg
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100 µg
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500 µg
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Applications*
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• Genomic DNA from whole blood, serum, plasma, cultured cells, buffy coat
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| * Kits to be used for research purposes only! |
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoBond® CB kits |
| NucleoBond® CB 20 |
20
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NucleoBond® AXG 20 Columns, buffers, Proteinase K |
740507 |
| NucleoBond® CB 100 |
20
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NucleoBond® AXG 100 Columns, buffers, Proteinase K |
740508 |
| NucleoBond® CB 500 |
10
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NucleoBond® AXG 500 Columns, buffers, Proteinase K |
740509 |
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| NucleoBond® AXG columns without buffers |
| NucleoBond® AXG 20 |
20
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NucleoBond® AXG 20 Columns |
740544 |
| NucleoBond® AXG 100 |
20
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NucleoBond® AXG 100 Columns |
740545 |
| NucleoBond® AXG 500 |
10
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NucleoBond® AXG 500 Columns |
740546 |
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| Product accessories |
Pack of
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Specification |
REF |
| NucleoBond® Small Rack |
1
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for use with NucleoBond® AXG 20 Columns |
740562 |
| NucleoBond® Large Rack |
1
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for use with NucleoBond® AXG 100 and 500 Columns |
740563 |
For ordering click here
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