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| NucleoSpin® RNA L |
Total RNA from cells and tissue
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rDNase and NucleoSpin® Filters included
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| Features |
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| Midi spin kit for the isolation of RNA of highest integrity |
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• Efficient removal of genomic DNA – rDNase included for on-column digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters L (shredders)
included
• Up to 600 μg ready-to-use RNA
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| Technology |
Silica-membrane technology |
| Format |
Midi spin columns |
| Sample material |
< 5 x 107 cultured cells,
< 1010 bacterial cells,
< 3 x 108 yeast cells,
< 200 mg tissue
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| Fragment size |
200 b |
| Typical yield |
180 µg from 107 HeLa cells,
620 µg from 4 x 107 HeLa cells
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| A260/A280 |
1.9–2.1 |
| Typical RIN (RNA integrity number) |
> 9 |
| Elution volume |
500 µL |
| Preparation time |
80 min/4 preps |
| Binding capacity |
700 µg |
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Applications**
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| • Total RNA isolation from cultured cells, tissue (standard protocol) |
• Support protocol for total RNA from < 1010 bacterial cells (Gram-negative, Gram-positive) or
< 3 x 108 yeast cells
• Support protocol for RNA clean-up from reaction mixtures
• Support protocol for total RNA from samples stored in RNAlater® |
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension,
array technology, RNase protection assays |
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* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA L system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
** Kits to be used for research purposes only
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoSpin® RNA L |
20
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NucleoSpin® RNA L Columns with Collection Tubes
(15 mL), Collection Tubes (15 mL), NucleoSpin® Filters L, buffers, RNase-free rDNase |
740962.20 |
For ordering click here
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