NucleoSpin® RNA L	Total RNA from cells and tissue
Features
Complete midi kit with recombinant DNase and shredders for DNA-free* RNA of high quality
• rDNase included for on-column digestion ® efficient removal of genomic DNA • NucleoSpin Filters L (shredders) included ® efficient homogenization and reduction of viscosity • Up to 600 µg ready-to-use RNA

  

Technology	Silica-membrane technology
Format	Midi spin columns
Sample material	< 5 x 107 cultured cells < 1010 bacterial cells, up to 3 x 108 yeast cells < 200 mg tissue
Fragment size	200 b - 20 kb
Typical yield	180 µg from 107 HeLa cells 620 µg from 4 x 107 HeLa cells
A260/280	1.9-2.1
Typical RIN (RNA integrity number)	> 9
Elution volume	500 µl
Preparation time	80 min/4 preps
Binding capacity	700 µg
Applications**
• Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from £ 1010 bacterial cells (Gram-negative and Gram-positive) • Support protocol for total RNA from £  3 x 108 yeast cells • Support protocol for RNA clean-up from reaction mixtures • Support protocol for total RNA from samples stored in RNAlater® • RNA suitable for e.g. real-time RT-PCR, Northern bloting, primer extension, array technology, RNase    protection assays
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The eventuality of DNA detection with PCR increases with: – the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected    with plasmid – decreasing PCR amplicon size ** Kits to be used for research purposes only