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| NucleoTrap® mRNA |
Poly(A) mRNA isolation from total RNA
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| Features |
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| For fast purification of poly(A) mRNA from total RNA |
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• High binding capacity: 5 µg poly(A) mRNA/mg oligo(dT) latex beads (20 µl bead suspension)
• Support protocol for direct mRNA isolation from cells available
• High quality poly(A) mRNA without degradation and DNA contamination
• Convenient and fast processing by microcentrifugation using NucleoTrap Microfilters
• mRNA purification in only 30 min
• Available in miniprep and midiprep format
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NucleoTrap mRNA Mini
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NucleoTrap mRNA Midi
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| Technology |
Affinity chromatography
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| Format |
Oligo(dT) latex-bead suspension
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| Sample material |
250 µg total RNA
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1000 µg total RNA
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| Fragment size |
50 b-20 kbp
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50 b-20 kbp
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| Typical yield |
10 µg mRNA
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40 µg mRNA
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| A260/280 |
1.9-2.1
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1.9-2.1
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| Elution volume |
10-20 µl
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10-20 µl
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| Preparation time |
30 min/6 preps
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30 min/6 preps
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| Binding capacity |
5 µg poly(A) mRNA/20 µl oligo(dT) latex-bead suspension
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Applications
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• Poly(A) mRNA isolation from total RNA
• Clean-up of in vitro transcripts
• Direct purification of poly(A) mRNA from cells (support protocol)
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Ordering information
| Product |
Preps
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Specification |
Reference |
| NucleoTrap mRNA Mini |
12
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NucleoTrap Microfilters, Microcentrifuge Tubes (2 ml),
24 mg Oligo(dT) Latex Beads, buffers
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740655 |
| NucleoTrap mRNA Midi |
12
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NucleoTrap Microfilters, Microcentrifuge Tubes (2 ml),
90 mg Oligo(dT) Latex Beads, buffers
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740656 |
For ordering click here
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