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| NucleoTrap® mRNA |
Poly(A) mRNA isolation from total RNA
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| Features |
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| For fast purification of poly(A) mRNA from total RNA |
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• High binding capacity: 5 μg poly(A) mRNA/mg oligo(dT) latex beads (20 μL bead suspension)
• Support protocol for direct mRNA isolation from cells available
• High quality poly(A) mRNA without degradation and DNA contamination
• Convenient and fast processing by microcentrifugation using NucleoTrap® Microfilters
• mRNA purification in only 30 min
• Available in mini and midi format
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NucleoTrap® mRNA Mini
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NucleoTrap® mRNA Midi
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| Technology |
Affinity chromatography
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| Format |
Oligo(dT) latex-bead suspension
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| Sample material |
250 µg total RNA
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1000 µg total RNA
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| Fragment size |
50 b–20 kbp
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50 b–20 kbp
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| Typical yield |
10 µg mRNA
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40 µg mRNA
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| A260/A280 |
1.9–2.1
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1.9–2.1
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| Elution volume |
10–20 µL–
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10–20 µL
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| Preparation time |
30 min/6 preps
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30 min/6 preps
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| Binding capacity |
5 µg poly(A) mRNA/20 µL oligo(dT) latex-bead suspension
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Applications*
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• Poly(A) mRNA isolation from total RNA
• Clean-up of in-vitro transcripts
• Direct purification of poly(A) mRNA from cells (support protocol)
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* Kits to be used for research purposes only
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Ordering information
| Product |
Preps
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Specification |
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| NucleoTrap® mRNA Mini |
12
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NucleoTrap® Microfilters, Microcentrifuge Tubes (2 mL),
Oligo(dT) Latex Beads, buffers
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740655 |
| NucleoTrap® mRNA Midi |
12
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NucleoTrap® Microfilters, Microcentrifuge Tubes (2 mL),
Oligo(dT) Latex Beads, buffers
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740656 |
For ordering click here
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