NucleoSpin® RNA Plant	Total RNA from plant
Features
Complete plant mini kit with recombinant DNase and shredders for DNA-free* RNA of high quality
• Two alternative lysis buffers included ® optimal lysis conditions for all species • rDNase included for on-column digestion ® efficient removal of genomic DNA • NucleoSpin Filters (shredders) included ® efficient homogenization and reduction    of viscosity • Up to 70 µg ready-to-use RNA • Parallel purification of genomic DNA possible by using the NucleoSpin RNA/DNA     Buffer Set

  

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	1 - 100 mg tissue
Fragment size	200 b - 20 kb
Typical yield	3 - 70 µg from 100 mg plant material
A260/280	1.9-2.1
Elution volume	60 µl
Preparation time	30 min/6 preps
Binding capacity	200 µg
Applications**
• Total RNA from plant cells and tissue • Total RNA from filamentous fungi • RNA suitable for e.g. real-time RT-PCR, Northern blotting, primer extension, array technology, RNase    protection assays
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Plant system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with: – the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid – decreasing PCR amplicon size ** Kits to be used for research purposes only