|
|
|
|
| NucleoSpin® 8/96 RNA |
Total RNA from cells and tissue
|
|
| Features |
|
| Time-saving isolation of total RNA for varying throughput in flexible 8-well strip format and for high throughput in approved 96-well format |
|
 • Time-saving parallel isolation of total RNA
• DNase included for efficient removal of genomic DNA
• Processing possible under vacuum or by centrifugation
• Suitable for manual and automated processing
• Innovative MN Wash Plate minimizes risk of cross-contamination
• RNA ready-to-use for any kind of enzymatic reaction
|
|
NucleoSpin 8 RNA
|
NucleoSpin 96 RNA
|
| Technology |
Silica-membrane technology
|
| Format |
8-well strips
|
96-well plates
|
| Processing |
Manual or automated, vacuum or centrifugation
|
| Sample material |
< 107 cultured cells (centrifugation)
< 30 mg tissue (centrifugation)
|
| Fragment size |
200 b - 20 kbp
|
200 b - 20 kbp
|
| Typical yield |
< 100 µg
|
< 100 µg
|
| A260/280 |
1.90 - 2.10
|
1.90 - 2.10
|
| Typical RIN (RNA integrity number) |
> 9 (cells)
³ 7 (tissue)
|
> 9 (cells)
³ 7 (tissue)
|
| RNA ratio |
28S/18S » 2.1
|
28S/18S » 2.1
|
| Typical concentration |
50 - 200 ng/µl
|
50 - 200 ng/µl
|
| Elution volume |
50 - 130 µl
|
50 - 130 µl
|
| Preparation time |
45 min/6 strips
|
70 min/plate
|
| Binding capacity |
100 µg
|
100 µg
|
|
|
Applications*
|
|
|
• Manual or automated isolation of total RNA from cultured cells and tissue
|
|
| * Kits to be used for research purposes only |
|
|
| |
|
|
Ordering information
| Product |
Preps
|
Specification |
Reference |
| NucleoSpin 8 RNA |
12 x 8
|
NucleoSpin RNA Binding Strips, MN Wash Plates, MN Square-well Blocks, Racks with Tube Strips, Cap Strips, Elution plates U-bottom, Self-adhering Foil, buffers, RNase-free DNase I |
740698 |
|
60 x 8
|
as above |
740698.5 |
| NucleoSpin 96 RNA |
2 x 96
|
NucleoSpin RNA Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block (low), Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free DNase I |
740709.2 |
|
4 x 96
|
as above |
740709.4 |
|
24 x 96
|
as above |
740709.24 |
|
|
|
|
| Product accessories |
Pack of
|
Specification |
Reference |
| NucleoVac 96 Vacuum Manifold |
1
|
|
740681 |
| NucleoVac Vacuum Regulator |
1
|
for controlling of vacuum |
740641 |
| Starter Set A |
1
|
for use of NucleoSpin 8-well strips on the NucleoVac 96 vacuum manifold |
740682 |
| Starter Set C |
1
|
for use of NucleoSpin 8-well strips under centrifugation |
740684 |
| NucleoSpin RNA Filter Plate |
4
|
96-well plates for filtration of cell and tissue homogenates, for use under vacuum or centrifugation |
740711 |
| NucleoSpin RNA Filter Strips |
12
|
8-well strips for filtration of cell and tissue homogenates, for use under vacuum or centrifugation |
740699.12F |
|
60
|
as above |
740699.60F |
For ordering click here
|
| |
|
|
 |