NucleoSpin 8 / 96 RNA

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NucleoSpin® 8/96 RNA

Application notes
Application notes NucleoSpin® 8/96 RNA

Use of MN Wash Plate

NucleoSpin^® 8 / 96 RNA
NucleoSpin^® 8 / 96 RNA Core Kit
Total RNA from cells and tissue

Features

Isolation of total RNA in flexible 8-well strip format and for high throughput
in approved 96-well format

• Time-saving parallel isolation of total RNA
• rDNase included for efficient removal of genomic DNA
• Processing under vacuum or by centrifugation
• Suitable for manual and automated processing
• Innovative MN Wash Plate minimizes risk of cross-contamination
• RNA ready to use for any kind of enzymatic reaction
• NucleoSpin® 8 / 96 RNA Core Kits: Kits with basic content focussed on automation platforms.
Additional accessories can be combined as needed.

NucleoSpin® 8 RNA
NucleoSpin® 8 RNA Core Kit

NucleoSpin® 96 RNA
NucleoSpin® 96 RNA Core Kit

Technology
Silica-membrane technology

Format
8-well strips

96-well plates

Processing
Manual or automated, vacuum or centrifugation

Sample material
< 107 cultured cells (centrifugation)
< 30 mg tissue (centrifugation)
saliva (collected with Oragene®)

Fragment size
> 200 b

> 200 b

Typical yield
< 100 µg

< 100 µg

A260/A280
1.90–2.10

1.90–2.10

Typical RIN (RNA integrity number)
> 9 (cells)
≥ 7 (tissue)

> 9 (cells)
≥ 7 (tissue)

RNA ratio
28S/18S approx. 2.1

28S/18S approx. 2.1

Typical concentration
50–200 ng/µL

50–200 ng/µL

Elution volume
50–130 µL

50–130 µL

Preparation time
45 min/6 strips

70 min/plate

Binding capacity
100 µg

100 µg

Applications*

• Manual or automated isolation of total RNA from cultured cells and tissue
• Total RNA from saliva samples collected with Oragene®•RNA (Genotek)

* Kits to be used for research purposes only

Ordering information

Product
Preps
Specification REF

NucleoSpin® 8 RNA
12 x 8
NucleoSpin® RNA Binding Strips, MN Wash Plates, MN Square-well Blocks, Racks of Tube Strips, Elution Plate U-bottom, Self-adhering Foil, buffers, RNase-free rDNase 740698

60 x 8
as above 740698.5

NucleoSpin® 8 RNA Core Kit
48 x 8
NucleoSpin® RNA Binding Strips, buffers, RNase-free rDNase 740465.4

NucleoSpin® 96 RNA
2 x 96
NucleoSpin® RNA Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase 740709.2

4 x 96
as above 740709.4

24 x 96
as above 740709.24

NucleoSpin® 96 RNA Core Kit
4 x 96

NucleoSpin® RNA Binding Plates, buffers, RNase-free rDNase
740466.4

Product accessories
Pack of
Specification REF

NucleoVac 96 Vacuum Manifold
1

740681

NucleoVac Vacuum Regulator
1
for controlling of vacuum 740641

Starter Set A
1
for use of NucleoSpin® 8-well strips on the NucleoVac 96 Vacuum Manifold 740682

Starter Set C
1
for use of NucleoSpin® 8-well strips under centrifugation 740684

NucleoSpin® RNA Filter Plate
4
96-well plates for filtration of cell and tissue homogenates, for use under vacuum or centrifugation 740711

NucleoSpin® RNA Filter Strips
12
8-well strips for filtration of cell and tissue homogenates, for use under vacuum or centrifugation 740699.12F

60
as above 740699.60F

For ordering click here