Editors
     
  
     
  
     
NucleoSpin® 8 / 96 RNA
NucleoSpin® 8 / 96 RNA Core Kit

RNA from cells and tissue

Features
Isolation of RNA – flexible 8-well strip format for varying throughput and proven 96-well format for high throughput
• Time-saving parallel isolation of total RNA
• rDNase included for efficient removal of genomic DNA
• Processing under vacuum or by centrifugation
• Suitable for manual and automated processing
• Innovative MN Wash Plate minimizes risk of cross-contamination
• RNA ready to use for any kind of enzymatic reaction
• NucleoSpin® 8 / 96 RNA Core Kits: Kits with basic content focussed on automation platforms.
   Additional accessories can be combined as needed.

NucleoSpin® 8 RNA
NucleoSpin® 8 RNA Core Kit

NucleoSpin® 96 RNA
NucleoSpin® 96 RNA Core Kit

Technology Silica-membrane technology
Format 8-well strips

96-well plates

Processing Manual or automated, vacuum or centrifugation
Sample material < 107 cultured cells (centrifugation)
< 30 mg tissue (centrifugation)
saliva (collected with Oragene®)
Fragment size > 200 nt

> 200 nt

Typical yield

20 µg (2 x 106 HeLa cells),
20 µg (20 mg mouse liver)

20 µg (2 x 106 HeLa cells),
20 µg (20 mg mouse liver)

A260/A280 1.90–2.10

1.90–2.10

Typical RIN (RNA integrity number) > 9 (cells)
 7 (tissue)

> 9 (cells)
7 (tissue)

RNA ratio 28S/18S approx. 2.1

28S/18S approx. 2.1

Typical concentration 50–200 ng/µL

50–200 ng/µL

Elution volume 50–130 µL

50–130 µL

Preparation time 45 min/6 strips

70 min/plate

Binding capacity 100 µg

100 µg

Applications*

• Manual or automated isolation of total RNA from cultured cells and tissue
• RNA from saliva samples collected with Oragene®•RNA (Genotek)
* Kits to be used for research purposes only
  
Maximize    

     

Ordering information

Product

Preps

Specification REF
NucleoSpin® 8 RNA

12 x 8

NucleoSpin® RNA Binding Strips, MN Wash Plates, MN Square-well Blocks, Racks of Tube Strips, Elution Plate U-bottom, Self-adhering Foil, buffers, RNase-free rDNase 740698

60 x 8

as above 740698.5
NucleoSpin® 8 RNA Core Kit

48 x 8

NucleoSpin® RNA Binding Strips, buffers, RNase-free rDNase 740465.4
NucleoSpin® 96 RNA

2 x 96

NucleoSpin® RNA Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Block Low, Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase 740709.2

4 x 96

as above 740709.4
NucleoSpin® 96 RNA Core Kit

4 x 96

NucleoSpin® RNA Binding Plates, buffers, RNase-free rDNase

740466.4
Related products

Preps

Specification REF
NucleoSpin® 96 RNA Trace

4 x 96

for small sample amounts
< 5 x 105 cultured cells, < 2 mg human / animal tissue
NucleoSpin® RNA Trace Binding Plates, MN Wash Plates, Square-well Blocks, Elution Plates U-bottom, buffers, RNase-free rDNase
740731.4
NucleoSpin® 8 RNA Tissue Core Kit 48 x 8 for larger  tissue samples
> 30 mg human / animal tissue
NucleoSpin® RNA Tissue Binding Strips, buffers, RNase-free rDNase
740732.4
NucleoSpin® 96 RNA Tissue Core Kit

4 x 96

for larger  tissue samples
> 30 mg human / animal tissue
NucleoSpin® RNA Tissue Binding Plates, buffers, RNase-free rDNase
740733.4
Product accessories

Pack of

Specification REF
NucleoVac 96 Vacuum Manifold

1


740681
NucleoVac Vacuum Regulator

1

for controlling of vacuum 740641
Starter Set A

1

for use of NucleoSpin® 8-well strips on the NucleoVac 96 Vacuum Manifold 740682
Starter Set C

1

for use of NucleoSpin® 8-well strips under centrifugation 740684
NucleoSpin® RNA Filter Plate

4

96-well plates for filtration of cell and tissue homogenates, for use under vacuum or centrifugation 740711
NucleoSpin® RNA Filter Strips

12

8-well strips for filtration of cell and tissue homogenates, for use under vacuum or centrifugation 740699.12F

60

as above 740699.60F

For ordering click here

  
   
  
Copyright 2018 by MACHEREY-NAGEL GmbH & Co. KG   Imprint / Impressum Privacy StatementTerms Of Use