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| NucleoBond® RNA/DNA |
Total RNA from cells and tissue
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| Features |
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| Anion-exchange chromatography - extra high purity for up to 400 µg total RNA |
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• Ultra-pure RNA from different samples
• Separate isolation of different RNA species (tRNA, rRNA, mRNA) possible
• Separate elution of genomic DNA possible
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NucleoBond
RNA/DNA 80
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NucleoBond
RNA/DNA 400
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| Technology |
Anion-exchange chromatography
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| Format |
Midi gravity-flow columns
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Maxi gravity-flow columns
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| Sample material |
5 x 106 cultured eucaryotic cells
20 mg tissue
0.5x108 bacteria /yeast cells
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2 x 107 eucaryotic cells
100 mg tissue
2x109 bacteria /yeast cells
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| Fragment size |
500 bp - 300 kbp
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500 bp - 300 kbp
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Typical RNA yield
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70 µg from 5 x 106 cultured cells
30 µg from 20 mg tissue
50 µg from 0.5x108 bacteria
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300 µg from 2 x 107 cultured cells
150 µg from 100 mg tissue
200 µg from 2x109 bacteria
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| A260/280 |
1.8-1.95
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1.8-1.95
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| Preparation time |
1.5-2.5 h
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1.5-2.5 h
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| Binding capacity |
80 µg
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400 µg
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Applications*
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• Total RNA from cultured cells, tissue, bacteria
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| * Kits to be used for research purposes only |
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Ordering information
| Product |
Preps
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Specification |
Reference |
| NucleoBond RNA/DNA 80 |
25
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NucleoBond AXR 80 Columns, buffers |
740650 |
| NucleoBond RNA/DNA 400 |
10
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NucleoBond AXR 400 Columns, buffers |
740651 |
For ordering click here
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