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| NucleoBond® RNA/DNA |
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| Features |
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Anion-exchange chromatography – extra high purity for up to 400 μg total RNA
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• Ultra-pure RNA from different samples
• Separate isolation of different RNA species (tRNA, rRNA, mRNA) possible
• Separate elution of genomic DNA possible
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NucleoBond®
RNA/DNA 80
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NucleoBond®
RNA/DNA 400
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| Technology |
Anion-exchange chromatography
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| Format |
Midi gravity-flow columns
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Maxi gravity-flow columns
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| Sample material |
5 x 106 cultured eukaryotic cells,
20 mg tissue,
5 x 107 bacteria / yeast cells
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2 x 107 cultured eukaryotic cells,
100 mg tissue,
2 x 109 bacteria / yeast cells
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| Fragment size |
50 b–300 kb
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50 b–300 kb
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Typical RNA yield
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70 μg from 5 x 106 cultured cells,
30 μg from 20 mg tissue,
50 μg from 5 x 107 bacteria
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300 μg from 2 x 107 cultured cells,
150 μg from 100 mg tissue,
200 μg from 2 x 109 bacteria
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| A260/A280 |
1.80–1.95
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1.80–1.95
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| Preparation time |
1.5–2.5 h
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1.5–2.5 h
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| Binding capacity |
80 µg
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400 µg
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Applications*
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• Total RNA from cultured cells, tissue, bacteria
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| * Kits to be used for research purposes only |
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Ordering information
| Product |
Preps
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Specification |
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| NucleoBond® RNA/DNA 80 |
25
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NucleoBond® AXR 80 Columns, buffers |
740650 |
| NucleoBond® RNA/DNA 400 |
10
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NucleoBond® AXR 400 Columns, buffers |
740651 |
For ordering click here
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