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NucleoBond® RNA/DNA

  
     
NucleoBond® RNA/DNA
Features
Anion-exchange chromatography – extra high purity for up to 400 μg RNA

 

• Ultra-pure RNA from different samples
• Separate isolation of different RNA species (tRNA, rRNA, mRNA) possible
• Separate elution of genomic DNA

NucleoBond® RNA/DNA 80

NucleoBond® RNA/DNA 400

Technology Anion-exchange chromatography
Format Midi gravity-flow columns Maxi gravity-flow columns
Sample material 5 x 106 cultured eukaryotic cells,
20 mg tissue,
5 x 107 bacteria / yeast cells

2 x 107 cultured eukaryotic cells,
100 mg tissue,
2 x 109 bacteria / yeast cells

Fragment size 50 nt–300 knt

50 nt–300 knt

Typical RNA yield

70 μg from 5 x 106 cultured cells,
30 μg from 20 mg tissue,
50 μg from 5 x 107 bacteria

300 μg from 2 x 107 cultured cells,
150 μg from 100 mg tissue,
200 μg from 2 x 109 bacteria

A260/A280 1.80–1.95

1.80–1.95

Preparation time 1.5–2.5 h

1.5–2.5 h

Binding capacity 80 µg 400 µg

Applications*

• RNA from cultured cells, tissue, bacteria
* Kits to be used for research purposes only
  
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Ordering information

Product

Preps

Specification REF
NucleoBond® RNA/DNA 80

25

NucleoBond® AXR 80 Columns, buffers 740650
NucleoBond® RNA/DNA 400

10

NucleoBond® AXR 400 Columns, buffers 740651

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