NucleoSpin® RNA II	Total RNA from cells and tissue
rDNase included, NucleoSpin Filters included
Features
Complete mini kit with recombinant DNase and shredders for DNA-free* RNA of high quality
• DNase included for on-column digestion ® efficient removal of genomic DNA • NucleoSpin Filters (shredders) included ® efficient homogenization and reduction    of viscosity • Up to 70 µg ready-to-use RNA • Parallel purification of genomic DNA possible by using the NucleoSpin RNA/DNA     Buffer Set

  

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 5 x 106 cultured cells < 109 bacterial cells, up to 108 yeast cells < 30 mg tissue
Fragment size	> 200 b
Typical yield	14 µg from 106 HeLa cells 70 µg from 109 bacterial cells
A260/280	1.9-2.1
Typical RIN (RNA integrity number)	> 9
Elution volume	40-100 µl
Preparation time	30 min/6 preps
Binding capacity	200 µg
Applications**
• Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from paraffin embedded tissue • Support protocol for total RNA from £ 109 bacterial cells (Gram-negative and Gram-positive) • Support protocol for total RNA from £ 108 yeast cells • Support protocol for total RNA from £ 100 µl biological fluids • Support protocol for RNA clean-up from reaction mixtures • Support protocol for total RNA from samples stored in RNAlater®
• RNA suitable for e.g. real-time RT-PCR, Northern blotting, primer extension, array technology, RNase    protection assays
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA II system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with: – the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid – decreasing PCR amplicon size. ** Kits to be used for research purposes only