NucleoSpin® RNA II	Total RNA from cells and tissue
rDNase included, NucleoSpin® Filters included
Features
Mini spin kit for the isolation of RNA of highest integrity
• Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion* • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders)    included • Up to 70 μg ready-to-use RNA • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set

  

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 5 x 106 cultured cells, < 109 bacterial cells, < 108 yeast cells, < 30 mg tissue
Fragment size	> 200 b
Typical yield	14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells
A260/A280	1.9–2.1
Typical RIN (RNA integrity number)	> 9
Elution volume	40–120 µL
Preparation time	30 min/6 preps
Binding capacity	200 µg
Applications**
• Total RNA isolation from cultured cells, tissue (standard protocol)
• Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells • Support protocol for total RNA from ≤ 100 μL biological fluids • Support protocol for RNA clean-up from reaction mixtures • Support protocol for total RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,    RNase protection assays
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA II system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with: – the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid – decreasing PCR amplicon size ** Kits to be used for research purposes only