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NucleoMag® DNA Plasma

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NucleoMag® DNA Plasma

  
     
NucleoMag® DNA Plasma

Isolation of cell-free DNA from plasma samples

Features
Magnet bead based isolation of cell-free DNA from 1–10 mL blood plasma
• Consistent cfDNA recovery from 1–10 mL plasma samples
• Efficient purification of fragmented DNA as small as 50 bp
• No PCR inhibition regardless of your preferred sample volume
Technology Magnetic-bead technology
Format Highly reactive superparamagnetic beads
Processing

Manual or automated

Sample material

1–10 mL human EDTA/Cell-Free DNA BCT® plasma

Fragment size ³ 50 bp
Typical yield Depending on sample source, storage, and quality
A260/A280 1.6–1.9
Elution volume 50–200 µL
Preparation time 55 min/24 preps (2 mL; excl. lysis)*
Binding capacity 0.3 µg/µL beads

Applications**

• Manual and automated isolation of circulating cell-free DNA from human plasma, derived from e.g., EDTA
   blood draw tubes or Cell-Free DNA BCT® for “Liquid Biopsy” analysis**
• Typical downstream applications: NGS, qPCR, ddPCR
* Established on a KingFisher® Flex
** Kits to be used for research purposes only!
  
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Ordering information

Product

Preps

Specification REF
NucleoMag® DNA Plasma

1 x 48

NucleoMag® B-Beads, buffers, Liquid Proteinase K 744550.1

4 x 48

as above 744550.4
Material to be supplied by the user
E.g. 24-Square-well Block with silicone lid (lysis plate)

4

24-well blocks with 10 mL flat bottom wells 740679.4
E.g. 24-Square-well Block
U-bottom (separation plate)

24

24-well blocks with 10 mL square wells 740448.24
E.g. Elution Plate U-bottom (elution plate)

24

96-well microplates with 300 µL u-bottom wells 740486.24

 

For use with KingFisher® platform

 

KingFisher® 24 Accessory Kit

1 set

24 Deep-well Blocks, 24-Tip Combs, and Plates for 5 x 24 preparations 744953
KingFisher® Duo Prime Accessory Kit B 1 set 24 Deep-well Blocks, 6-Tip Combs, and Plates for 8 x 6 preparations 744954
Product accessories

Pack of

Specification REF
NucleoMag® SEP 24

1

magnetic separator 744903
NucleoMag® SEP Maxi 1 magnetic separator 744902
Snap Tubes (50 mL) 10 50 mL tubes 740822.10
50 as above 740822.50
Liquid Proteinase K 5 mL 740396

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