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NucleoZOL

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NucleoZOL

  
     
 
NucleoZOL

RNA from cells and tissue

  No chloroform – 
no phase separation

Features 
The universal reagent for RNA isolation
• No chloroform, no phase separation, easy procedure
• High RNA yield and purity from any sample material
• Small and large RNA in one or in separated fractions
Technology One-phase extraction
Format Reagent
Sample material

< 1 x 107 cultured cells, bacteria, and yeast,
< 100 mg human/animal/plant tissue,
< 0.4 mL (viral) fluids

Fragment size Small RNA (10–200 nt). Large RNA (> 200 nt)
Typical yield (total RNA)



Typical yield (large RNA)

Liver: 6-8 µg/mg tissue
Kidney, spleen: 3-4 µg/mg tissue
Muscle, brain, lung: 0.5-1.5 µg/mg
Cultured cells: 4-10 µg/106 cells
Liver: 5-7 µg/mg tissue
Kidney, spleen: 3-4 µg/mg tissue
Muscle, brain, lung: 0.5-1.5 µg/mg
Cultured cells: 3-8 µg/106 cells

A260/A280 1.8–2.1
Typical RIN (RNA integrity number) > 9
Elution volume Flexible
Preparation time < 1 h

Applications*

• RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

* Reagent to be used for research purposes only

  
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Ordering information

Product

Pack of

Specification REF
NucleoZOL

200 mL

NucleoZOL reagent 740404.200

Related products Preps Specification REF
NucleoSpin® RNA Clean-up 10 NucleoSpin® RNA Clean-up Columns with Collection Tubes,  Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers 740948.10
50 as above 740948.50
250 as above 740948.250
NucleoSpin® RNA Clean-up XS 10

NucleoSpin® RNA Clean-up XS Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers

740903.10
50 as above 740903.50
250 as above 740903.250
NucleoSpin® RNA Set for NucleoZOL 50  NucleoSpin® RNA Columns, Collection Tubes, buffers 740406.50

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