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RNA purification
Do it right! RNA purification by MACHEREY-NAGEL

 

 

RNA isolation is greatly complicated by the presence of ubiquitous RNases that degrade RNA samples. Furthermore, the physical and biochemical structure of sample materials makes RNA purification more difficult and even RNA extraction from tissue or blood samples can be a challenging task.

The world of RNA analysis switched to the specific detection of different RNA species, e.g., mRNA, miRNA, siRNA, and new technologies such as RNA sequencing increase the demand on high quality RNA extraction kits. 

Why choose MN for your RNA application?

Over the years, MN Bioanalysis has gained vast experience in RNA isolation and thus developed into highly skilled RNA experts. The MN research and the technical support team are current in all RNA applications (RNA sequencing, qPCR, microarrays, etc.). This has enabled MN to provide high-value RNA purification protocols in view of the expansive research applications.
We invite you to take advantage of our excellent RNA purification kits as well as our team of scientific experts from MACHEREY-NAGEL.

RNA from cells and tissue
Format Sample Product
Mini spin columns < 30 mg tissue,
< 5 x 107 cultured cells
NucleoSpin® RNA
Mini spin columns < 30 mg tissue,
< 1 x 106 cultured cells
NucleoSpin® RNA Plus
Reagent < 100 mg tissue,
< 1 x 107 cultured cells,
< 0.4 mL (viral) fluids
NucleoZOL
Mini Spin columns < 500 µL NucleoZOL sample
NuceloSpin® RNA Set for NucleoZOL
XS spin columns < 5 mg tissue,
1–105 cultured cells
NucleoSpin® RNA XS
Midi spin columns < 200 mg tissue,
< 5 x 107 cultured cells
NucleoSpin® RNA Midi
8-well strips < 20 mg tissue,
< 107 cultured cells
NucleoSpin® 8 RNA / Core Kit
96-well plates < 20 mg tissue,
< 107 cultured cells
NucleoSpin® 96 RNA / Core Kit
Flexible < 20 mg tissue,
< 2 x 106 cultured cells
NucleoMag® 96 RNA
Micro RNA
Format Sample Product
Mini spin columns < 107 cultured cells,
< 30 mg tissue,
< 50 mg plant tissue,
< 150 μL reaction mixture
NucleoSpin® miRNA
Mini spin columns < 300 µL plasma, serum
(< 900 µLwith multiple loading steps)
NucleoSpin® miRNA Plasma
Solution serum, plasma Exosome Precipitation Solution (Serum/Plasma)
Solution urine Exosome Precipitation Solution (Urine)
RNA, DNA, and protein
Format Sample Product
Mini spin columns < 30 mg tissue,
< 100 mg plant tissue,
< 5 x 106 cultured cells
NucleoSpin® TriPrep
Mini spin columns < 30 mg tissue,
< 100 mg plant tissue,
< 5 x 106 cultured cells
NucleoSpin® RNA/Protein
Buffer set < 30 mg tissue,
< 100 mg plant tissue,
< 5 x 106 cultured cells
NucleoSpin® RNA/DNA Buffer Set
RNA from blood
Format Sample Product

Mini spin columns < 200 µL whole blood
(< 400 µL with two loading steps) 
NucleoSpin® RNA Blood

Midi spin columns 400–1300 µL whole blood NucleoSpin® RNA Blood Midi
8-well strips < 400 µL whole blood NucleoSpin® 8 RNA Blood
96-well plates < 400 µL whole blood NucleoSpin® 96 RNA Blood
RNA from plant
Format Sample Product
Mini spin columns < 100 mg (wet weight),
< 20 mg (dry weight) plant tissue
NucleoSpin® RNA Plant
Small and large RNA
Format Sample Product
Mini spin columns 4–10 sections (á 10 μm, á up to
 6 cm2 tissue area), < 50 mg of tissue
NucleoSpin® totalRNA FFPE
XS spin columns 1–3 sections (á 10 μm, á up to
6 cm2 tissue area), < 5 mg of tissue
NucleoSpin® totalRNA FFPE XS
Poly(A) mRNA from total RNA
Format Sample Product
Latex beads 250–1000 μg total RNA NucleoTrap® mRNA
RNA clean-up
Format Sample Product
Mini spin columns < 200 µL phenol / chloroform extract or reaction mixture
           
NucleoSpin® RNA Clean-up
XS columns < 300 μL RNA solution containing
< 90 μg RNA
NucleoSpin® RNA Clean-up XS
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