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| NucleoSpin® RNA Blood |
RNA from whole blood
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| Features |
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| Isolation of RNA from fresh or frozen whole blood |
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• Direct total blood lysis: Very simple and convenient procedure
(no selective erythrocyte lysis – complete processing at room temperature)
• Outstanding RNA yield from up to 400 µL whole blood
• Efficient on-column DNA digest for an increased sensitivity in downstream applications
• Compatible with common blood collection tubes and anticoagulants
(e.g., EDTA, citrate, and heparin)
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
Up to 400 µL whole blood (fresh or frozen)
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| Fragment size |
> 200 nt |
| Typical yield |
1.2–8 µg (400 µL whole blood)*
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| Typical purity (A260/A280) |
1.9–2.1 |
| Elution volume |
40–120 µL |
| Preparation time |
55 min/6 preps |
| Binding capacity |
200 µg |
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| * RNA yield strongly depends on the leukocyte number in each individual blood sample. |
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Applications**
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• Isolation of RNA from fresh or frozen whole blood from common blood collection tubes
(EDTA, citrate, heparin) |
| • Typical downstream applications: qRT-PCR, Next generation sequencing, blotting, array technology |
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** Kits to be used for research purposes only
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoSpin® RNA Blood |
10
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NucleoSpin® RNA Blood Columns, Collection Tubes (2 mL), buffers, RNase-free rDNase, Liquid Proteinase K |
740200.10 |
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50
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as above |
740200.50 |
For ordering click here
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