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| Substances: |
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DOPAC; serotonin; dopamine; epinephrine; norepinephrine; DOPA; DOPS |
| Column: |
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EC 100/4 NUCLEOSHELL HILIC, 2.7 µm
EC 250/4 NUCLEODUR® HILIC, 3 µm |
| Phase: |
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NUCLEOSHELL HILIC
NUCLEODUR® HILIC |
| REF number: |
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763334.40
760530.40 |
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| Sample pretreatment: |
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each compound 1 mg/mL in eluent
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| Conditions: |
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NUCLEODUR® HILIC
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NUCLEOSHELL HILIC |
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Eluent:
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acetonitrile – 100 mmol/L ammonium formate, pH 3.2
(80:20, v/v) |
| Flow rate : |
1 mL/min |
1 mL/min |
4 mL/min |
| Temperature : |
25 °C |
| Pressure : |
116 bar |
95 bar |
395 bar |
| Injection volume : |
0.5 µL |
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| Detection: |
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UV, 280 nm |
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| Author(-s): |
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HPLC department
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| Source: |
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MACHEREY-NAGEL, Germany 2011
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| Keywords: |
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core-shell; ultra-fast HPLC
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Chromatogram:
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 |
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| Legend: |
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blue chromatogram - NUCLEOSHELL HILIC with flow rate of 4 mL/min
turqois chromatogram - NUCLEOSHELL HILIC with flow rate of 1 mL/min
orange chromatogram - NUCLEODUR® HILIC with flow rate of 1 mL/min
1 - DOPAC
2 - serotonin
3 - dopamine
4 - epinephrine
5 - norepinephrine
6 - DOPA
7 - DOPS |
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| Note: |
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Under identical conditions, the separation sequence on NUCLEOSHELL HILIC is comparable with the sequence on NUCLEODUR® HILIC. It can also be seen that the separation on core-shell phase takes place in a shorter time. With further acceleration the separation on NUCLEOSHELL HILIC can be shortened to 1 min.
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