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NucleoBond® Xtra BAC
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Transfection-grade plasmid DNA
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| Features |
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The 2nd generation of anion exchanger for large construct plasmid DNA
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• Optimized column design: one prep in approximately 75 min
• Column filter included: parallel lysate clearing and loading onto the column
• Optimal silica material: yields up to 150 μg
• Transfection-grade plasmid DNA using proven anion-exchange technology
• LyseControl: visualization of a completed lysis / neutralization
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| Technology |
Anion-exchange chromatography
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| Format |
Gravity flow columns
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| Lysate clarification |
Column filters
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| Sample material |
250–750 mL
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Vector size
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< 300 kbp
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| Typical yield |
10–100 µg
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| A260/A280 |
1.80–1.95
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| Isopropanol precipitation |
Centrifugation
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| Preparation time |
75 min/prep
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| Binding capacity |
150 µg |
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| Applications |
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• Purification of P1, BACs, PACs, and other large constructs from E. coli
• Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoBond® Xtra BAC |
10
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NucleoBond® Xtra BAC Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A |
740436.10 |
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25
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as above |
740436.25 |
| Product accessories |
Pack of
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Specification |
REF |
| NucleoBond® Xtra Combi Rack |
1
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for use with NucleoBond® Xtra Midi, Maxi, BAC, AX 100, 500, 2000, and 10000 Columns |
740415 |
| NucleoBond® Xtra BAC Buffer Set |
1
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additional lysis buffers (1 L each): Buffers RES-BAC, LYS-BAC, NEU-BAC, and RNase A |
740437 |
For ordering click here
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