Comparison of ALUGRAM® Xtra SIL G UV254 and ALUGRAM® Xtra Nano-SIL G UV254 for the separation of peppermint oil ingredients and japanese herbal oil ingredients

Method:	Matrix	Application-No.:
TLC		403640

Substances:		menthol; further ingredients of peppermint oil; further ingredients of japanese herbal oil
Plate:		ALUGRAM® Xtra Nano-SIL G UV254, 20 x 20 cm ALUGRAM® Xtra SIL G UV254, 20 x 20 cm
Phase:		Xtra Nano-SIL G UV254 Xtra SIL G UV254
REF number:		818343 818333
Sample pretreatment:		Sample solutions: 0.1 mL of oil are dissolved in 3 mL toluene Reference solution: 10 mg menthol are solved in 1 mL toluene
Conditions:		Applied volume: 2 μl per track Eluent: n-hexane – ethyl acetate (9.7:0.3, v/v) Separation time: 7 min
Detection:		after developing and drying, the plate is immersed in anisaldehyde reagent (0.5 mL anisaldehyde, 10 mL acetic acid, 85 mL methanol, 5 mL sulfuric acid) and then heatened at 100 °C (oven, heating plate)
Author(-s):		TLC department
Source:		MACHEREY-NAGEL, Germany 2011
Keywords:		comparison; japanese herbal oil; peppermint oil
Chromatogram:
Legend:		1  -  peppermint oil, 2 µL 2  -  menthol standard, 2 µL 3  -  japanese herbal oil, 2 µL
Note:		By using nanoparticles on ALUGRAM® Xtra Nano-SIL G UV254 migration distance decreases compared to ALUGRAM® Xtra SIL G/UV254. But the spots on ALUGRAM® Xtra Nano-SIL G UV254 are more focused.

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