Flash RS Tech Support

CHROMABOND® Flash RS cartridges
for Flash separations from 10 mg up to 160 g
Technical support

	Technical features
	distribution of the eluent stream via highly porous frits cartridge material organic solvent resistant, low bleed polypropylene, thick column walls, one-piece body, sophisticated length to diameter ratio for high plate numbers and excellent separation efficiency
	Cartridge/column connections
	CHROMABOND® RS cartridges are 100 % compatible with the ISCO® Companion®, no additional hardware is needed for this type of purification systems. CHROMABOND® RS cartridges (except RS 800 and RS 1600 with Maxi Luers) can also be used as stand alone system with any pump/detector/fraction collector combination using the CHROMABOND® Flash Starter Kit (REF 730798) or the CHROMABOND® Flash Stand Alone Kit (REF 732903).
	For the RS 800 and RS 1600 we offer stand alone adaptors Maxi Luer to ¼”-28 screws: Column inlet: Maxi Luer connector, male maxi luer to ¼”-28 inner screw, stainless steel, single use product (REF 732900, left) Column exit: Aluminium bridge with stainless steel screw for Maxi Luer Output, female Maxi luer to ¼”-28 inner screw for RS 800 or RS 1600, respectively (REF 732901/732902, right)

Loadability			Loadability table
Due to the narrow particle size distribution, the excellent packing quality and the optimised stationary phases (acid washed silica, reduced particulate matter) our cartridges can realize highest loadability at best possible separation efficiency. Additionally, the large range of different cartridge lengths and diameters eases to find the optimum in loadability for a given sample amount.			SiOH cartridge	Average loadability / cartridge [g]
				difficult separation	easy separation
			RS 4	0.04	0.4
			RS 15	0.15	1.5
			RS 25	0.25	2.5
			RS 40	0.4	4
			RS 80	0.8	8
Rule of thumb for the loadability			RS 120	1.2	12
separation	loadability	g sample / g adsorbent	RS 200	2	20
difficult	low	≤ 1 %	RS 330	3.3	33
easy	high	≤ 10 %	RS 800	8	80
			RS 1600	16	160

Back pressure / pressure stability
The back pressure always depends on flow rate and viscosity of the eluent mixture, column length and diameter and particle size. The new ultra performance CHROMABOND® Flash RS cartridges up to 200 g silica are stable up to 15 bar (220 psi), > 200 g: 12 bar (176 psi)		We recommend a pressure guard, because short time pressure peaks (viscosity of eluent or change in gradient) can exceed the pressure limit.

Back pressure of CHROMABOND® SiOH Flash cartridges for the eluent hexane – ethyl acetate 9:1 or 8:2
	Flow rate
Cartridge	20 mL/min	40 mL/min	80 mL/min	120 mL/min	160 mL/min	200 mL/min	240 mL/min
RS 4	0.75 bar	1.5 bar
RS 15	0.25 bar	0.75 bar	1.5 bar	2.0 bar
RS 25	0.5 bar	1.0 bar	1.75 bar	3.0 bar	4.0 bar	5.0 bar
RS 40		0.75 bar	1.5 bar		3.0 bar		3.5 bar
RS 80			1.5 bar	2.5 bar	3.0 bar	3.5 bar	4.0 bar
RS 120			1.0 bar	1.5 bar	2.0 bar	2.5 bar	3.0 bar
RS 200			1.0 bar		2.0 bar		3.0 bar
RS 330			1.5 bar		3.0 bar		4.0 bar

Conditioning volumes for CHROMABOND® Flash cartridges (normally 1.5 column volumes of eluent)
Cartridge	Eluent volume for conditioning	Cartridge	Eluent volume for conditioning
RS 4	20 mL	RS 120	440 mL
RS 15	60 mL	RS 200	750 mL
RS 25	90 mL	RS 330	1100 mL
RS 40	140 mL	RS 800	2900 mL
RS 80	280 mL	RS 1600	5000 mL

Upscaling of the optimum flow rate		Upscaling of flow rate
This depends on the eluent and the separation problem. For RS cartridges the upscaling relation is easy: silica [g] to flow = 1:1 (for the same polarity of eluent), e.g.,
4 g silica	optimum flow rate ~ 6–12 mL/min
40 g silica	optimum flow rate ~ 60–120 mL/min

TLC upscaling

TLC is often used for the development of a selective and reproducible method in Flash chromatography, because it is often necessary to test a large number of eluent and/or adsorbent combinations. MN TLC plates and sheets are coated with the same base silica, which is used in our CHROMABOND® Flash cartridges. This is an important prerequisite for the reproducible transfer of a TLC separation to the Flash column, because the parameters are identical in both systems.

How can a successful TLC separation be transferred to a Flash column?
MN as a TLC manufacturer uses the same base material/silica on TLC plates as in Flash cartridges:

same selectivities and easy upscaling from TLC to Flash is guaranteed

saves time and money, because expensive optimisations are not necessary

Examples:
Rf values of the TLC separation should be in the range of 0.1–0.4 (low height).

ΔRf values on the TLC plate should be as high as possible.

OK		× not OK

		eluent polarity too high for optimal transfer

OK		× not OK

		insufficient Flash separation to be expected

During TLC optimisation always use solvents, which are well suited for the following Flash chromatography!

Separation efficiency and reproducibility tests
Our optimised and automatic packing process leads to an excellent packing quality, irrespective of the phase or particle size distribution (normal phase or reversed phase, spherical or irregular particles). MN, as a manufacturer of silica, has decades of experience in the production of first class separation phases and columns. This leads to highest separation efficiencies of the columns, a constant back pressure (via controlled narrow particle size distribution) and good reproducibility from cartridge to cartridge.		The separation efficiency is in the first step not influenced by the dimension or the geometry of the Flash RS cartridges. The chromatograms below show an identical resolution and peak form for different column dimensions, when flow and sample amount is adjusted correctly. This is positive for optimization and upscaling experiments.
Resolution and peak shape for different column dimensions

MN adsorbents · a unique variety of phases
	All Flash columns and cartridges from MN are available with our whole range of CHROMABOND® phases (more than 40, e.g., C18, C8, OH, Alox etc.) Technical specification for modified and plain silica phases: acid-washed irregular silica, pore size 60 Å, particle size 45 µm, specific surface 500 m2/g, pH stability 2–8,
	Additionally you can choose from our range of irregular POLYGOPREP silica packings in particle sizes from 20 to 130 μm and pore sizes from 60 to 4000 Å
	For high performance Flash separations you can order columns packed with spherical NUCLEODUR® featuring very high separation efficiency and extremely increased column lifetime (particle size > 20 μm)
	For corresponding offers please contact your local MN distributor.