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NucleoSpin® Soil

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NucleoSpin® Soil

Sample homogenization
NucleoSpin® Bead Tubes

  
     
NucleoSpin® Soil

Isolation of genomic DNA from soil and sediments

Alternative lysis options –
get highest yield
Features
Isolation of total DNA from diverse soil types

• Two alternative lysis buffers and a special additive (Enhancer SX) for optimal 
   processing of various soil samples – high yield and high purity
• NucleoSpin® Bead Tubes with ceramic beads for most efficient cell disruption
   of Gram-positive and Gram-negative bacteria, archaea, yeast, fungi, and algae in soil, 
   sludge,or sediment
• NucleoSpin® Inhibitor Removal Column to eliminate all PCR inhibitors,
   such as humic substances – DNA is ready-to-use for PCR without dilution

Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 500 mg soil, sludge, or sediment

Typical yield 2–10 µg (500 mg soil)
Elution volume 30–100 µL
Preparation time 90 min/10 preps
Binding capacity 50 µg
Applications
• Total DNA from microorganisms in soil and sediment
• Quantify and detect microorganisms from environmental samples
• Typical downstream applications: PCR, real-time PCR, Southern blotting, microarray technology

 

  
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References

PLoS One  2013 July;8(7):e67699. doi:101371/journal.pone.0067699.
Comparison of DNA Extraction Methods in Analysis of Salivary Bacterial Communities
Lazarevic V, Gaia N, Girard M, Francois P, Schrenzel J.

View PUBLICATION

  
     

Ordering information

Product

Preps

Specification REF
NucleoSpin® Soil

10

NucleoSpin® Soil Columns, NucleoSpin® Bead Tubes, NucleoSpin® Inhibitor Removal Columns, Collection Tubes (2 mL), Collection Tubes (2 mL, lid), buffers 740780.10

50

as above 740780.50

250

as above 740780.250

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