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| NucleoSpin® FFPE RNA/DNA |
RNA and DNA from FFPE samples
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| Features |
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| Parallel isolation of RNA and DNA from formalin-fixed, paraffin-embedded samples |
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• Get RNA and DNA from the same FFPE specimen – without sample splitting
• Safe time, money, and precious sample material
• Very easy paraffin removal – Paraffin Dissolver (patent pending) included
• Enhanced RT-PCR performance – high decrosslinking efficiency
• High quality DNA – ready to use for genotyping
• Higher sensitivity with concentrated RNA – elution in as little as 5 µl
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns-XS design |
| Sample material |
Up to 7 sections (10 µm) of 250 mm2 total area,
or up to 15 mg paraffin1)
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| RNA/DNA yield |
Depending on amount and quality of the sample |
| Typical RIN (RNA integrity number) |
2 – 6 (Strongly depending on sample quality)
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| Elution volume RNA |
5 – 30 µl |
| Elution volume DNA |
10 – 30 µl |
| Binding capacity |
90 µg RNA |
| Maximum column loading volume |
600 µl |
| 1) When using the standard protocol with Paraffin Dissolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the standard protocol with xylene for deparaffinization. |
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Applications*
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• Rapid isolation of RNA and DNA, or RNA from formalin-fixed, paraffin-embedded samples
e.g., colon carcinoma (unstained); colon carcinoma (hematoxylin stained); colon mucosa,
adenocarcinoma of colon transversum, liver (rat / human), liver carcinoma (hematoxylin stained),
lymph node, spleen (rat / human); pancreas (rat / human; diabetic / non-diabetic), placenta
• Isolation of RNA and DNA, or RNA from fresh and archived FFPE samples
• Isolation of RNA and DNA, or RNA from specimen on object slides (stained or unstained)
• RNA ready to use in RT-PCR, DNA ready to use in PCR
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* KITS TO BE USED FOR RESEARCH PURPOSES ONLY. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.
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Ordering information
| Product |
Preps
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Specification |
Reference |
| NucleoSpin FFPE RNA/DNA |
10
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NucleoSpin FFPE Columns, Paraffin Dissolver, Buffers, Proteinase K, rDNase (RNase-free), Collection Tubes (2 ml), Collection Tubes (1.5 ml), User Manual |
740978.10 |
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50
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as above |
740978.50 |
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250
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as above |
740978.250 |
For ordering click here
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