Login
     
  
     

Literature

User Manual 
NucleoSpin
FFPE RNA/DNA

  
     

NucleoSpin® FFPE RNA/DNA

RNA and DNA from FFPE samples

Features 
Parallel isolation of RNA and DNA from formalin-fixed, paraffin-embedded samples

Get RNA and DNA from the same FFPE specimen – without sample splitting
• Safe time, money, and precious sample material
• Very easy paraffin removal – Paraffin Dissolver (patent pending) included
• Enhanced RT-PCR performance – high decrosslinking efficiency
• High quality DNA – ready to use for genotyping
• Higher sensitivity with concentrated RNA – elution in as little as 5 µl

  
Technology Silica-membrane technology
Format Mini spin columns-XS design
Sample material

Up to 7 sections (10 µm) of 250 mm2 total area,
or up to 15 mg paraffin1)

RNA/DNA yield Depending on amount and quality of the sample
Typical RIN (RNA integrity number)

2 – 6 (Strongly depending on sample quality)

Elution volume RNA 5 – 30 µl
Elution volume DNA 10 – 30 µl
Binding capacity 90 µg RNA
Maximum column loading volume 600 µl
1) When using the standard protocol with Paraffin Dissolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the standard protocol with xylene for deparaffinization.

Applications*

• Rapid isolation of RNA and DNA, or RNA from formalin-fixed, paraffin-embedded samples
   e.g., colon carcinoma (unstained); colon carcinoma (hematoxylin stained); colon mucosa,
   adenocarcinoma  of colon  transversum, liver (rat / human), liver carcinoma (hematoxylin stained),
   lymph node, spleen (rat / human); pancreas (rat / human; diabetic / non-diabetic), placenta
• Isolation of RNA and DNA, or RNA from fresh and archived FFPE samples
• Isolation of RNA and DNA, or RNA from specimen on object slides (stained or unstained)
• RNA ready to use in RT-PCR, DNA ready to use in PCR

KITS TO BE USED FOR RESEARCH PURPOSES ONLY. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.

  
Maximize    

     

Ordering information

Product

Preps

Specification Reference
NucleoSpin FFPE RNA/DNA

10

NucleoSpin FFPE Columns, Paraffin Dissolver, Buffers, Proteinase K, rDNase (RNase-free), Collection Tubes (2 ml), Collection Tubes (1.5 ml), User Manual 740978.10

50

as above 740978.50

250

as above 740978.250

For ordering click here

  
     
  
Copyright 2009 by MACHEREY-NAGEL GmbH & Co. KG   Imprint / Impressum Privacy StatementTerms Of Use