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| NucleoSpin® FFPE RNA/DNA |
RNA and DNA from FFPE samples
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| Features |
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| Parallel isolation of RNA and DNA from formalin-fixed, paraffin-embedded samples |
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• Get RNA and DNA from the same FFPE specimen – without sample splitting
• Save time, money, and precious sample material
• Very easy paraffin removal – Paraffin Dissolver (patent pending) included
• Enhanced RT-PCR performance – high decrosslinking efficiency
• High quality DNA – ready to use for genotyping
• Higher sensitivity with concentrated RNA – elution in as little as 5 μL
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns-XS design |
| Sample material |
≤ 7 sections (10 μm) of 250 mm2 total area (< 15 mg paraffin*)
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| Typical yield |
Depending on amount and quality of the sample |
| Typical RIN (RNA integrity number) |
Strongly depending on sample quality (2–6)
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| Elution volume RNA |
5–30 µL |
| Elution volume DNA |
10–30 µL |
| Preparation time |
90 min/6 preps (plus 3 h to overnight for lysis) |
| Binding capacity |
110 µg RNA |
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| * When using the standard protocol with Paraffin Dissolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the standard protocol with xylene for deparaffinization. |
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Applications*
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• Rapid isolation of RNA and DNA, or RNA only from formalin-fixed, paraffin-embedded samples,
e.g., colon carcinoma (unstained), colon carcinoma (hematoxylin stained), colon mucosa, adenocarcinoma
of colon transversum, liver (rat / human), liver carcinoma (hematoxylin stained), lymph node, spleen
(rat / human), pancreas (rat / human; diabetic / non-diabetic), placenta
• Isolation of RNA and DNA, or RNA only from fresh and archived FFPE samples
• Isolation of RNA and DNA, or RNA only from specimen on object slides (stained or unstained)
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| • RNA ready to use in RT-PCR, DNA ready to use in PCR |
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* KITS TO BE USED FOR RESEARCH PURPOSES ONLY. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoSpin® FFPE RNA/DNA |
10
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NucleoSpin® FFPE Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), Paraffin Dissolver, buffers, Proteinase K, RNase-free rDNase
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740978.10 |
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50
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as above |
740978.50 |
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250
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as above |
740978.250 |
For ordering click here
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