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NucleoSpin®
FFPE RNA/DNA

  
     

NucleoSpin® FFPE RNA/DNA

RNA and DNA from FFPE samples


Features 

Parallel isolation of RNA and DNA from formalin-fixed, paraffin-embedded samples

 

 

• Get RNA and DNA from the same FFPE specimen – without sample splitting
• Save time, money, and precious sample material
• Very easy paraffin removal – Paraffin Dissolver (patent pending) included
• Enhanced RT-PCR performance – high decrosslinking efficiency
• High quality DNA – ready to use for genotyping
• Higher sensitivity with concentrated RNA – elution in as little as 5 μL

  
Technology Silica-membrane technology
Format Mini spin columns-XS design
Sample material

7 sections (10 μm) of 250 mm total area (< 15 mg paraffin*)

Typical yield Depending on amount and quality of the sample
Typical RIN (RNA integrity number)

Strongly depending on sample quality (2–6)

Elution volume RNA 5–30 µL
Elution volume DNA 10–30 µL
Preparation time 90 min/6 preps (plus 3 h to overnight for lysis)
Binding capacity 110 µg RNA

* When using the standard protocol with Paraffin Dissolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the standard protocol with xylene for deparaffinization.

Applications*


 • Rapid isolation of RNA and DNA, or RNA only from formalin-fixed, paraffin-embedded samples,
   e.g., colon carcinoma (unstained), colon carcinoma (hematoxylin stained), colon mucosa, adenocarcinoma
   of colon transversum, liver (rat / human), liver carcinoma (hematoxylin stained), lymph node, spleen
   (rat / human), pancreas (rat / human; diabetic / non-diabetic), placenta
• Isolation of RNA and DNA, or RNA only from fresh and archived FFPE samples
• Isolation of RNA and DNA, or RNA only from specimen on object slides (stained or unstained)

• RNA ready to use in RT-PCR, DNA ready to use in PCR

KITS TO BE USED FOR RESEARCH PURPOSES ONLY. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.


 

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® FFPE RNA/DNA

10

NucleoSpin® FFPE Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), Paraffin Dissolver, buffers, Proteinase K, RNase-free rDNase

740978.10

50

as above 740978.50

250

as above 740978.250

For ordering click here

  
     
  
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