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| Substances: |
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naphthol yellow S and further ingredients
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| Plate: |
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ALUGRAM® Xtra SIL G UV 254, 20 x 20 cm |
| Phase: |
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Xtra SIL G UV 254 |
| REF number: |
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818333
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| Sample pretreatment: |
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Humidify 10 mg drug with 50 µl water in a small glass reaction tube. After 3 minutes add 1 ml methanol and store the solution for 20 minutes in the dark. Afterwards filter through a CHROMAFIL Xtra GF-100/25
filter.
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| Conditions: |
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Applied volume: 10 μl
Eluent: ethyl acetate / 2-propanol / water (65:25:10 v/v/v)
Migration distance: 10 cm
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| Detection: |
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The sheet is dried with a hair dryer and analysed under daylight, UV 254 nm and UV 366 nm.
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| Author(-s): |
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MACHEREY-NAGEL |
| Source: |
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TLC department, Germany 2010
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| Keywords: |
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saffron ingredients
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Chromatogram:
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| Note: |
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The chromatograms show as main compound naphthol yellow S. Other coloured zones may appear.
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