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Separation of 18 PAHs on NUCLEODUR® C18 PAH
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Method:
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Matrix
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Application-No.:
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HPLC
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123840
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| Substances: |
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naphthalene; acenaphthylene; 1-methylnaphthalene; 2-methylnaphthalene; acenaphthene; fluorene; phenanthrene; anthracene; fluoranthene; pyrene; benz[a]anthracene; chrysene; benzo[b]fluoranthene; benzo[k]fluoranthene; benzo[a]pyrene; dibenz[ah]anthracene; benzo[ghi]perylene; indeno[1,2,3-cd]pyrene |
| Column: |
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EC 125/4 NUCLEODUR® C18 PAH, 3 µm
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| Phase: |
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NUCLEODUR® C18 PAH |
| REF number: |
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760784.40 |
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Samples:
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Concentrations:
UV, 10 ng/µl
Fluorescence, 10 ng/µl
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| Conditions: |
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Eluent A: methanol / water (70:30, v/v)
Eluent B: acetonitrile
Gradient: 0-20 % B in 1.5 min, 20-50 % B in 1.5 min, 50-100 % B in 1.0 min, 100 % B for 3.0 min, 100 - 0 % B in 0.5 min
Flow rate: 1.5 ml/min
Temperature: 35 °C
Injection volumes:
UV, 1 µl (10 ng per compound)
Fluorescence, 0.5 µl (5 ng per compound)
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| Detection: |
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UV, 254 nm
Fluorescence, see chromatogram
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| Author(-s): |
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HPLC department
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| Source: |
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MACHEREY-NAGEL, Germany 2010
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| Keywords: |
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18 PAHs; EPA
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Chromatogram:
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| Legend: |
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Chromatogram (blue) - UV detection
Chromatogram (red) - Fluorescence detection
1 - naphthalene
2 - acenaphthylene (not detectable with fluorescence)
3 - 1-methylnaphthalene
4 - 2-methylnaphthalene
5 - acenaphthene
6 - fluorene
7 - phenanthrene
8 - anthracene
9 - fluoranthene
10 - pyrene
11 - benz[a]anthracene
12 - chrysene
13 - benzo[b]fluoranthene
14 - benzo[k]fluoranthene
15 - benzo[a]pyrene
16 - dibenz[ah]anthracene
17 - benzo[ghi]perylene
18 - indeno[1,2,3-cd]pyrene |
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Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications. |
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