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Fast Separation of 16 EPA PAHs on NUCLEODUR® C18 PAH
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Method:
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Matrix
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Application-No.:
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HPLC
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123820
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| Substances: |
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naphthalene; acenaphthylene; acenaphthene; fluorene; phenanthrene; anthracene; fluoranthene; pyrene; benz[a]anthracene; chrysene; benzo[b]fluoranthene; benzo[k]fluoranthene; benzo[a]pyrene; dibenz[ah]anthracene; benzo[ghi]perylene; indeno[1,2,3-cd]pyrene |
| Column: |
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EC 100/4 NUCLEODUR® C18 PAH, 3 µm
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| Phase: |
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NUCLEODUR® C18 PAH |
| REF number: |
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760783.40 |
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| Conditions: |
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Eluent A: methanol / water (80:20, v/v)
Eluent B: acetonitrile
Gradient: 2-20 % B in 1.2 min, 20-100 % B in 0.5 min, 100 % B for 2.5 min, 100-2 % B in 0.4 min, 2 % B for 5 min (equilibration)
Flow rate: 2.5 ml/min
Temperature: 35 °C
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| Detection: |
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A: UV, 254 nm
B: Fluorescence, see chromatogram
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| Author(-s): |
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HPLC department
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| Source: |
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MACHEREY-NAGEL, Germany 2010
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| Keywords: |
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EPA; PAHs; fast separation |
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Chromatogram:
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| Legend: |
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1 - Naphthalene
2 - Acenaphthylene (not detectable with fluorescence)
3 - Acenaphthene
4 - Fluorene
5 - Phenanthrene
6 - Anthracene
7 - Fluoranthene
8 - Pyrene
9 - Benz[a]anthracene
10 - Chrysene
11 - Benzo[b]fluoranthene
12 - Benzo[k]fluoranthene
13 - Benzo[a]pyrene
14 - Dibenz[ah]anthracene
15 - Benzo[ghi]perylene
16 - Indeno[1,2,3-cd]pyrene |
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Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications. |
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