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Instruction leaflet:
NUCLEODUR®
RP columns


  
     
NUCLEODUR® C18 HTec
High purity silica phase for preparative HPLC
Nonpolar high density monomeric octadecyl modification with high capacity · endcapped · 18 % C · USP L1
Properties
Key features:
Reliable and durable standard RP phase for up-scaling to preparative scale, suited for LC/MS
High loadability and excellent stability
Outstanding base deactivation
Technical characteristics:
High density octadecyl modification
Pore size 110 Å; particle sizes 1.8 µm, 3 µm, 5 μm, 7 μm and 10 μm for analytical and preparative separations
Carbon content 18 %, pH stability 1–11
Recommended application:
Sophisticated analytical and preparative separations of basic, neutral and acidic pharmaceuticals, derivatised amino acids, pesticides, fat-soluble vitamins, aldehydes, ketones and phenolic compounds
For applications click here.


Standard program* of columns packed with NUCLEODUR® C18 HTec
Column system Particle size: 1.8 µm 3 µm 5 µm 7 µm 10 µm
Analytical EC columns
(standard system, 2–4.6 mm)
   
Preparative VarioPrep columns (VP, ≥ 8 mm)    
  if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.

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EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water
Length -> 30 mm 50 mm 75 mm 100 mm 125 mm 150 mm 250 mm
NUCLEODUR® C18 HTec, 1.8 µm
2 mm ID 760301.20 760305.20 760304.20 760306.20   760308.20  
3 mm ID 760301.30 760305.30   760306.30      
4 mm ID 760301.40 760305.40   760306.40      
4.6 mm ID 760301.46 760305.46   760306.46      
EC guard columns*   4 x 2 mm 761925.20   4 x 3 mm 761925.30  
NUCLEODUR® C18 HTec, 3 µm
2 mm ID   760321.20   760323.20 760324.20 760325.20 760326.20
3 mm ID   760321.30   760323.30 760324.30 760325.30 760326.30
4 mm ID   760321.40   760323.40 760324.40 760325.40 760326.40
4.6 mm ID   760321.46 760322.46 760323.46 760324.46 760325.46 760326.46
EC guard columns*   4 x 2 mm 761926.20   4 x 3 mm 761926.30  
CC guard columns**   8 x 3 mm 761120.30   8 x 4 mm 761120.40  
NUCLEODUR® C18 HTec, 5 µm
2 mm ID   760311.20   760313.20 760314.20 760315.20 760316.20
3 mm ID   760311.30   760313.30 760314.30 760315.30 760316.30
4 mm ID   760311.40   760313.40 760314.40 760315.40 760316.40
4.6 mm ID   760311.46 760312.46 760313.46 760314.46 760315.46 760316.46
EC guard columns*   4 x 2 mm 761927.20   4 x 3 mm 761927.30  
CC guard columns**   8 x 3 mm 761110.30   8 x 4 mm 761110.40  
Guard columns in packs of 3, EC columns in packs of 1.

Guard columns for EC columns   EC columns with ID Use guard
column holder
      2 mm 3 mm 4 mm 4.6 mm
* Column Protection System EC guard column 4/2 4/3 4/3 4/3 REF 718966
** ChromCart® guard column system CC guard column 8/3 8/3 8/4 8/4 REF 721359
Custom-packed EC columns with other dimensions are available on request.
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VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water
Length 10 mm ID 21 mm ID 32 mm ID 40 mm ID 50 mm ID
NUCLEODUR® C18 HTec, 5 µm
50 mm 762551.100 762551.210      
100 mm   762553.210 762553.320   762553.500
125 mm 762554.100 762554.210      
150 mm     762555.320 762555.400 762555.500
250 mm 762556.100 762556.210 762556.320 762556.400 762556.500
NUCLEODUR® C18 HTec, 7 µm
50 mm 762561.100 762561.210      
100 mm   762563.210 762563.320   762563.500
125 mm 762564.100 762564.210      
150 mm     762565.320 762565.400 762565.500
250 mm 762566.100 762566.210 762566.320 762566.400 762566.500
NUCLEODUR® C18 HTec, 10 µm
50 mm 762571.100 762571.210      
100 mm   762573.210 762573.320   762573.500
125 mm 762574.100 762574.210      
150 mm     762575.320 762575.400 762575.500
250 mm 762576.100 762576.210 762576.320 762576.400 762576.500
Guard columns with NUCLEODUR® C18 HTec
  8 mm ID 16 mm ID 32 mm 50 mm
10 mm * 762591.80 762591.160      
15 mm *     762592.320 762592.500
* VP guard column Size Pack of for VP column required holder REF
VP 10/8 10 x 8 mm DI 2 10 mm DI 8 mm 718251
VP 10/16 10 x 16 mm DI 2 21 mm DI 16 mm 718256
VP 15/32 15 x 32 mm DI 1 32–40 mm DI 32 mm 718253
VP 15/50 15 x 50 mm DI 1 50 mm DI 50 mm 718255
Custom-packed VP columns with other dimensions are available on request.
For details of the VarioPrep column system click here.
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NUCLEODUR® C18 HTec bulk packings

Ordering information

Phase Particle Size Pack of 100 g Pack of 1 kg
NUCLEODUR C18 HTec, 5 µm 5 µm 713830.0100 713830.1
NUCLEODUR C18 HTec, 7 µm 7 µm 713831.0100 713831.1
NUCLEODUR C18 HTec, 10 µm 10 µm 713832.0100 713832.1
Packed columns see above
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