NucleoSpin® miRNA	Small and large RNA isolation
microRNA extraction without phenol/chloroform
Features*
Parallel isolation of small and large RNA
• Superior selectivity - purify your RNA fractionated by size ... highest flexibility!    Isolation of small RNA only (18 – 200 bases) OR    Isolation of small (18 – 200 bases) and large (> 200 bases) RNA in two fractions OR    Isolation of total RNA (small and large RNA in one fraction)
• Additional isolation of the total protein fraction ® ready-to-use for analysis in SDS-PAGE and Western Blot    ... confirm RNA results on protein level!
• Excellent RNA recovery — even without phenol/chloroform ... less hazardous!    Lysis with chaotropic salt ® less hazardous    Spin-column based procedure ® easy-to-use
• Convenient handling — complete equipment provided ... ease of use!    NucleoSpin Filters ® efficient sample homogenization    NucleoSpin Protein Removal Columns ® highest purity of small RNA    rDNase ® highly efficient on-column digestion of genomic DNA at RT

  

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 107 cultured cells, < 30 mg human/animal tissue < 50 mg plant tissue, < 150 µl reaction mixture
Fragment size small RNA	18 – 200 bases
Fragment size large RNA	> 200 bases
Typical yield	10 µg small RNA, 95 µg large RNA from 107 HeLa cells
Binding capacity	200 µg
Elution volume	30 – 100 µl
Preparation time	< 45 min (6 preps human/animal tissue, small and large RNA) < 35 min (6 preps human/animal tissue, small RNA only)
Applications**
• Parallel isolation of small and large RNA from human/animal tissue and cultured cells • Parallel isolation of small and large RNA from plant tissue • Parallel isolation of small and large RNA in combination with phenol/chloroform (e.g., TRIzol®) lysis • Purification of siRNA and large dsRNA from DICER reactions
• RNA suitable for real-time RT-PCR, Northern Blot, chip hybridization, etc.
*   Patent pending
** Kits to be used for research purposes only