NucleoSpin® miRNA	Small and large RNA isolation
No phenol / chloroform; additional protein isolation
Features*
Parallel isolation of small and large RNA
• RNA purification fractionated by size: Isolation of small RNA only (< 200 b),    Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions    Isolation of total RNA (small and large RNA in one fraction) • Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis • Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform • NucleoSpin® Filters for efficient sample homogenization • rDNase for efficient on-column removal of genomic DNA

  

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 107 cultured cells, < 30 mg human / animal tissue < 50 mg plant tissue, < 150 µL reaction mixture
Fragment size	Small RNA: < 200 b, large RNA: > 200 b
Typical yield	10 µg small RNA, 95 µg large RNA from 107 HeLa cells
Elution volume	30–100 µL
Preparation time	< 45 min (6 preps human / animal tissue, small and large RNA) < 35 min (6 preps human / animal tissue, small RNA only)
Binding capacity	200 µg
Applications**
• Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue,    and in combination with phenol / chloroform (e.g., TRIzol®) lysis • Purification of siRNA and large dsRNA from DICER reactions
• Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization
*   Patent pending
** Kits to be used for research purposes only