NucleoSpin® TriPrep	Total RNA, DNA and protein isolation
NucleoSpin Filters, rDNase, Protein Solving Buffer included
Features
Parallel isolation of RNA, DNA, and protein from unsplit samples
• Convenient one-column preparation of RNA, DNA, and protein • Complete kit, including:       - NucleoSpin Filters (shredders) for optimal lysis     - rDNase for on-column DNA digestion    - Protein Solving Buffer for solving all types of proteins • High quality RNA and DNA, ready-to-use for downstream applications • High protein yield independent of protein size, localization, modification, etc. • Easy protein quantification using the Protein Quantification Assay

  

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	£ 5 x 106 cells* £ 30 mg human/animal tissue* £ 100 mg plant tissue*
DNA yield RNA yield Protein yield	£ 6 µg £ 70 µg £ 1200 µg
A260/A280 DNA A260/A280 RNA	1.7-1.9 1.9-2.1
Typical RIN (RNA integrity number)	Depending on sample quality: good quality samples (e.g. fresh HeLa cells or mouse liver) typically result in RIN >9
Elution volume DNA Elution volume RNA Resolubilization volume protein	100 µl 40-120 µl 10-100 µl
Preparation time RNA and DNA Preparation time protein	45 min/6 preps 35 min/6 preps
Binding capacity (RNA)**	200 µg
Applications***
• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for    different isolations necessary • Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping • Reliable interpretation of RNA, DNA, and protein amounts • Good preservation of protein primary structure and posttranslational modifications (e.g. protein    phosphorylation) allows protein analysis without additional inhibitors (e.g. proteinase or phosphatase    inhibitors) • Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast,    and plants • RNA and DNA are ready to use for all typical downstream applications • Proteins are ready-to-use for SDS-PAGE/Western-Blotting
* For samples larger than approximately 1 x 106 cells or 5 mg human/animal tissue, DNA yield does not increase linearly with the sample amount. DNA yield may decrease utilizing high sample amounts like 5 x 106 cells or 30 mg tissue. However, DNA yield will be sufficient for PCR analysis. ** Binding capacity of DNA ≤10 µg, strongly depending on RNA amount bound to the membrane. *** KITS TO BE USED FOR RESEARCH PURPOSES ONLY. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.