NucleoSpin® TriPrep	Total RNA, DNA and protein isolation
NucleoSpin® Filters, rDNase, Protein Solving Buffer included
Features
Parallel isolation of RNA, DNA, and protein from undivided samples
• Convenient one-column preparation of RNA, DNA, and protein • High quality RNA and DNA, ready to use for downstream applications • High protein yield independent of protein size, localization, modification, etc. • Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay • Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis,    rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins

  

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	≤ 5 x 106 cells	≤ 30 mg human/animal tissue	≤ 100 mg plant tissue
	Total RNA	Total DNA	Total protein
Fragment size	> 200 b	< 30 kbp	15–300 kbp
Typical yield	< 70 µg	< 6 µg	< 1200 µg
A260/A280	1.9–2.1	1.7–1.9	–
Typical RIN (RNA integrity number)	> 9	–	–
Elution volume (RNA and DNA) Resolubilization volume (protein)	40–120 µL	100 µL	10–100 µL
Preparation time	30 min/6 preps	35 min/6 preps (RNA+DNA)	30 min/6 preps
Binding capacity	200 µg	10 µg*	–
Applications**
• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for    different isolations necessary • Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping • Reliable interpretation of RNA, DNA, and protein amounts • Good preservation of protein primary structure and posttranslational modifications (e.g., protein    phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase    inhibitors) • Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and    plants • RNA and DNA are ready to use for all typical downstream applications • Proteins are ready to use for SDS-PAGE / Western blotting
* Binding capacity of DNA ≤ 10 μg, strongly depending on RNA amount bound to the membrane. ** Kits to be used for research purposes only. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.