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| Substances: |
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melamine; cyanuric acid |
| Column: |
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EC 125/2 NUCLEODUR® HILIC, 5 µm
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| Phase: |
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NUCLEODUR® HILIC |
| REF number: |
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760551.20 |
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| Sample concentration: |
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melamine: 10 µg/ml
cyanuric acid: 490 µg/ml |
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| Conditions: |
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Eluent: acetonitrile / 10 mM ammonium formiate, pH 4 (90:10, v/v)
Flow rate: 0.2 ml/min
Temperature: 25 °C
Injection volume: 2.0 µl
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| Detection: |
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a) UV, 210 nm
b) LC-MS:
melamine
Mode: ESI+
Sample temp.: 400 °C
Needle: 3.0 kV
Cone voltage: 50 V
cyanuric acid
Mode: ESI-
Sample temp.: 400 °C
Needle: 3.0 kV
Cone voltage: 40 V
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| Author(-s): |
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HPLC department |
| Source: |
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MACHEREY-NAGEL, Germany 2009 |
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| Keywords: |
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toxic compounds; food; HILIC; LC-MS |
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Chromatogram:
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a) UV detection
b) LC-MS detection
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