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Separation of Catecholamines on NUCLEODUR® HILIC

Method:
Matrix
Application-No.:
HPLC

123030




Substances:
norephedrine; dopamine; adrenaline; L-DOPA
Column:
EC 250/4 NUCLEODUR® HILIC, 3 µm
Phase:
NUCLEODUR® HILIC
REF number:
760530.40



Sample:
Concentration: 30 ng/µl



Conditions:

Eluent: acetonitrile / 25 mM ammonium formate, pH 3 (75:25, v/v)
Flow rate: 0.8 ml/min
Temperature: 25 °C
Injection volume: 5 µl




Detection:
UV, 218 nm



Author(-s):
HPLC department
Source:
MACHEREY-NAGEL, Germany 2009



Keywords:
catecholamines; HILIC






Chromatogram:









Legend:
1  -  norephedrine
2  -  dopamine
3  -  adrenaline
4  -  L-DOPA



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MACHEREY-NAGEL does not warrant the correctness of the applications.


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