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Separation of the Chemotherapeutic Agent 5-Fluorouracil
and Uracil on NUCLEODUR® HILIC


Method:
Matrix
Application-No.:
HPLC

123020




Substances:
5-fluorouracil (5-FU); uracil
Column:

EC 125/4 NUCLEODUR® HILIC, 3 µm

Phase:
NUCLEODUR® HILIC
REF number:
760531.40









Conditions:

Eluent: acetonitrile / 5 mM ammonium acetate (95:5, v/v)
Flow rate: 0.6 ml/min
Temperature: 25 °C




Detection:
UV, 254 nm



Author(-s):
HPLC department
Source:
MACHEREY-NAGEL, Germany 2009



Keywords:
chemotherapeutic agent; HILIC






Chromatogram:









Legend:
1  -  5-fluorouracil (5-FU)
2  -  uracil



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Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications.


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