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Separation of Acrylamide, Methacrylamide and Methacrylic Acid
on NUCLEODUR® HILIC

Method:
Matrix
Application-No.:
HPLC

123010




Substances:
acrylamide, methacrylamide; methacrylic acid
Column:

EC 125/4 NUCLEODUR® HILIC; 5 µm

Phase:
NUCLEODUR® HILIC
REF number:
760551.40









Conditions:

Eluent: acetonitrile / 0.1 % formic acid (98:2, v/v)
Flow rate: 0.6 ml/min
Temperature: 22 °C
Injection volume: 0.5 µl




Detection:
UV, 210 nm



Author(-s):
HPLC department
Source:
MACHEREY-NAGEL, Germany 2009



Keywords:
food; toxic compounds; HILIC






Chromatogram:









Legend:
1  -  methacrylamide
2  -  acrylamide
3  -  methacrylic acid



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Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications.


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