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| Substances: |
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creatinine; creatine
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| Column: |
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EC 125/2 NUCLEODUR® HILIC, 3 µm
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| Phase: |
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NUCLEODUR® HILIC |
| REF number: |
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760531.20 |
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| Sample: |
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Concentration: 30 ng/µL |
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| Conditions: |
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Eluent: acetonitrile / 10 mM ammonium acetate, pH 4 (70:30, v/v)
Flow rate: 0.2 mL/min
Temperature: 25 °C
Injection volume: 5 µL
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| Detection: |
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LC-MS
Mode: ESI+
Sample temp.: 450 °C
Needle: 3.0 kV
Cone voltage: 50 V
A separation with UV detection is shown by Appl. No.: 122990
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| Author(-s): |
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HPLC department |
| Source: |
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MACHEREY-NAGEL, Germany 2009 |
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| Keywords: |
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muscle metabolism; HILIC; LC-MS
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Chromatogram:
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| Legend: |
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1 - creatinine
2 - creatine |