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Separation of Aromatic Amino Acids and Histamine
on NUCLEODUR® HILIC

Method:
Matrix
Application-No.:
HPLC

122980




Substances:
phenylalanine; phenylglycine; tyrosine; histamine; histidine
Column:

EC 125/4 NUCLEODUR® HILIC, 3 µm

Phase:
NUCLEODUR® HILIC
REF number:
760531.40









Conditions:

Eluent: acetonitrile / 100 mM ammonium acetate, pH 4 (75:25, v/v)
Flow rate: 1.0 ml/min
Temperature: 25 °C
Injection volume: 0.5 µl




Detection:
UV, 218 nm



Author(-s):
HPLC department
Source:
MACHEREY-NAGEL, Germany 2009



Keywords:
amino acids; HILIC






Chromatogram:









Legend:
1  -  phenylalanine
2  -  phenylglycine
3  -  tyrosine
4  -  histamine
5  -  histidine



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MACHEREY-NAGEL does not warrant the correctness of the applications.


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