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| Separation of Water Soluble Vitamins on NUCLEODUR® HILIC |
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Method:
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Matrix
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Application-No.:
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HPLC
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122970
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| Substances: |
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nicotinamide (component of vitamin B3); vitamin B7 (vitamin H, biotin); vitamin B6 (pyridoxine, pyridoxal, pyridoxamine); vitamin C (ascorbic acid); vitamin B12 (cyanocobalamin); vitamin B1 (thiamine) |
| Column: |
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EC 125/4 NUCLEODUR® HILIC, 3 µm
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| Phase: |
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NUCLEODUR® HILIC |
| REF number: |
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760531.40 |
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| Conditions: |
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Eluent: A) acetonitrile; B) 25 mM ammonium acetate, pH4
Gradient: 80 % A for 1 min, 80-70 % A in 1 min, 70 % A for 11 min
Flow rate: 1.0 ml/min
Temperature: 25 °C
Injection volume: 30 µl
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| Detection: |
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UV, 254 nm |
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| Author(-s): |
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HPLC department |
| Source: |
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MACHEREY-NAGEL, Germany 2009 |
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| Keywords: |
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water soluble vitamins; HILIC |
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Chromatogram:
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| Legend: |
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1 - nicotinamide (component of vitamin B3)
2 - vitamin B7 (vitamin H, biotin)
3 - vitamin B6 (pyridoxine, pyridoxal, pyridoxamine)
4 - vitamin C (ascorbic acid)
5 - vitamin B12 (cyanocobalamin)
6 - vitamin B1 (thiamine) |
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Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications. |
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