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| Separation of Pyrimidine and Purine Bases on NUCLEODUR® HILIC |
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Method:
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Matrix
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Application-No.:
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HPLC
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122950
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| Substances: |
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thymine; uracil; adenine; cytosine; guanosine |
| Column: |
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EC 125/4 NUCLEODUR® HILIC, 5 µm
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| Phase: |
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NUCLEODUR® HILIC |
| REF number: |
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760551.40 |
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| Conditions: |
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Eluent: acetonitrile / 5 mM ammonium acetate (80:20, v/v)
Flow rate: 0.3 ml/min
Temperature: 25 °C
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| Detection: |
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UV, 254 nm |
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| Author(-s): |
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HPLC department |
| Source: |
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MACHEREY-NAGEL, Germany 2009 |
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| Keywords: |
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nucleic acid bases; HILIC |
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Chromatogram:
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| Legend: |
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1 - thymine
2 - uracil
3 - adenine
4 - cytosine
5 - guanosine |
| www.mn-net.com |
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Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications. |
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