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Separation of Organic Acids on NUCLEODUR® HILIC

Method:
Matrix
Application-No.:
HPLC

122930




Substances:

fumaric acid; oxalic acid; citric acid

Column:
EC 125/4 NUCLEODUR® HILIC, 3 µm
Phase:
NUCLEODUR® HILIC
REF number:
760531.40









Conditions:

Eluent: acetonitrile / 200 mM ammonium acetate, pH 6.8 (70:30, v/v)
Flow rate: 1.0 ml/min
Temperature: 25 °C
Injection volume: 0.5 µl




Detection:
UV, 220 nm



Author(-s):
HPLC department
Source:
MACHEREY-NAGEL, Germany 2009



Keywords:
organic acids; HILIC






Chromatogram:









Legend:
1  -  fumaric acid
2  -  oxalic acid
3  -  citric acid



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Concerning questions to literature applications please contact the cited author/s. Chemicals mentioned in applications are not manufactured or sold by MACHEREY-NAGEL. Applications mentioned in MACHEREY-NAGEL literature or databases are provided for informational purposes only. MACHEREY-NAGEL does not warrant that all applications have been tested in MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products.
MACHEREY-NAGEL does not warrant the correctness of the applications.


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