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Separation of nucleotides on NUCLEODUR® HILIC

Method:
Matrix
Application-No.:
HPLC

122920




Substances:
adenosine; cAMP; AMP; ADP; ATP
Column:

EC 125/4 NUCLEODUR® HILIC, 3 µm

Phase:
NUCLEODUR® HILIC
REF number:
760531.40









Conditions:

Eluent: acetonitrile – 100 mmol/L ammonium acetate, pH 5.3 (70:30, v/v)
Flow rate: 1.3 mL/min
Temperature: 25 °C
Injection volume: 0.5 µL




Detection:
UV, 259 nm



Author(-s):
HPLC department
Source:
MACHEREY-NAGEL, Germany 2009



Keywords:
nucleic acid bases; nucleotides; HILIC






Chromatogram:














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