Movie NucleoBond® Xtra Midi vs Qiagen® HiSpeed® Midi

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The NucleoBond Xtra motion picture shows experiments that have been performed according to each manufacturer’s protocol. The following kits and experimental parameters have been used:
Kits used:
MACHEREY-NAGEL: NucleoBond Xtra Midi Plus (Cat. No. 740412.10), in the motion picture named “MN”
QIAGEN®: HiSpeed® Plasmid Midi Kit (Qiagen® Cat. No. 12643), in the motion picture named “Q”
Experimental parameters:
Plasmid: pcDNA3.1, Bacterial strain: E.coli DH5a®, Culture medium: LB (Luria-Bertani), Cultivation conditions: Incubation over night at 37°C with constant shaking (200 rpm), OD600 of the overnight culture: 6.5, Volume of the overnight culture: 50 ml (each)

OD600 of the overnight culture as well as yield, purity, and concentration of the isolated plasmid DNA were determined by photometric measurement. The experiments have been performed under laboratory conditions.

RE-ENACTMENT OF THE PROCEDURE AND/OR ANY CHANGE IN EXPERIMENTAL CONDITIONS MAY LEAD TO DIFFERENT PERFORMANCE AND RESULTS.