Editors

Separation of Sugars with Acetone/Water as Mobile Phase

Method:
Matrix
Application-No.:
HPLC
122870

Substances:

fructose; glucose; sucrose (saccharose); maltose; lactose

Column:

EC 250/4 NUCLEODUR® 100-3 NH2-RP

Phase: NUCLEODUR® 100-3 NH2-RP
MN catalog number: on request
Conditions:

Eluent: acetone/water (80:20, v/v)
Flow rate: 0.8 ml/min
Temperature: 25 °C
Injection volume: 20 µl

Detection: RI

Author(-s): HPLC department
Source: MACHEREY-NAGEL, Germany 2009

Keywords:

sugars; amino propyl silanised phase; RI detection; without acetonitrile

Chromatogram:




Legend:

1  -  fructose
2  -  glucose
3  -  sucrose (saccharose)
4  -  maltose
5  -  lactose




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