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| Substances: |
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ketoprofen; fenoprop; fenoprofen; flurbiprofen; ibuprofen; carprofen; diclofenac; meclofenamic acid |
| Column: |
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CHROMABOND® HR-XA, 3 ml, 200 mg |
| Phase: |
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CHROMABOND® HR-XA |
| REF number: |
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730951 |
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| Sample pretreatment: |
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1 µg/ml pharmaceuticals in serum, adjusted on basic pH
with 1 N NaOH
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| Conditions: |
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Column conditioning: 5 ml methanol, then 5 ml water
(Do not let run the column dry!)
Sample aspiration: The prepared sample is passed through the column by vacuum
Washing: With the following washing mixtures impurities are removed:
a) 2.5 ml water
b) 2.5 ml 0.1 N NaOH
c) 5.0 ml methanol
Drying: With nitrogen or air
Elution: Analytes are eluted with 5 ml methanol / 1 % formic acid
Evaporation to dryness and reconstitution with 1 ml of mobile phase from subsequent HPLC
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| Subsequent analysis: |
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HPLC determination of recovery rates with EC 125/4 NUCLEODUR® C18 Gravity,
5 µm (REF 760100.40) in reference to Appl. No 122840
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| Author(-s): |
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SPE department |
| Source: |
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MACHEREY-NAGEL, Germany 2009 |
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| Keywords: |
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acidic pharmaceuticals; serum; polymeric anion exchanger
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Recovery rates:
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HR-XA
|
Oasis MAX
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PS-OH-
|
|
ketoprofen
|
90 %
|
85 %
|
88 %
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|
fenoprop
|
104 %
|
123 %
|
70 %
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fenoprofen
|
98 %
|
69 %
|
60 %
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flurbiprofen
|
106 %
|
98 %
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69 %
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ibuprofen
|
88 %
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58 %
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38 %
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carprofen
|
69 %
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89 %
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85 %
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diclofenac
|
95 %
|
94 %
|
75 %
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meclofenamic acid
|
92 %
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93 %
|
45 %
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| Legend: |
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Competitor Oasis® MAX, 3 ml, 200 mg
CHROMABOND® PS-OH-, 3 ml, 200 mg (REF 730396) |
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