Protein Quantification Assay	Protein quantification
Features
Fast, sensitive, and convenient assay for protein quantification
• Highest sensitivity and flexibility • Reducing agent and detergent compatible • Fast procedure allows protein quantification in only 40 min • The perfect supplement for NucleoSpin TriPrep and NucleoSpin RNA/Protein

  

Format	Buffer and reagent set
Sample size	1-600 µl containing 0.6-200 µg protein (BSA equivalents)
Protein concentration	approx. 10-20,000 ng/µl
Sample type	protein solved in Protein Solving Buffer PSB, PLB, Laemmli buffer, or equivalent, preferable free of nucleic acids
Correlation coefficient	0.97-1.00
Wavelength for light extinction measurement	570 nm (530-700 nm)
Time	approx. 40 min
Applications
• Protein quantification assays in	- microplates - microcuvettes - semimicrocuvettes - low-volume photometer, e.g. NanoDrop™
• Protein quantification in Laemmli Buffer • Protein quantification in buffers containing up to 10% SDS • Protein quantification in buffers containing reducing agents (e.g. b-mercaptoethanol (715 mM),    TCEP (50 mM), or DTT) • Protein quantification in buffers containing brome phenol blue, e.g. 0.02%