Protein Quantification Assay	Protein quantification
Features
Fast, sensitive, and convenient assay for protein quantification
• Highest sensitivity and flexibility • Reducing agent and detergent compatible • Fast procedure allows protein quantification in only 40 min • The perfect supplement for NucleoSpin® TriPrep, NucleoSpin® RNA/Protein, and NucleoSpin® miRNA

  

Format	Buffer and reagent set
Sample size	< 600 µL containing 0.6–200 µg protein (BSA equivalents)
Protein concentration	Approx. 10–20,000 ng/µL
Sample type	Protein solved in Protein Solving Buffer PSB, PLB, Laemmli buffer, or equivalent, preferable free of nucleic acids
Correlation coefficient	0.97–1.00
Wavelength for light extinction measurement	570 nm (530–700 nm)
Time	Approx. 40 min
Applications
• Protein quantification assays in	- microplates - microcuvettes - semimicrocuvettes - low-volume photometer (e.g., NanoDrop™)
• Protein quantification in Laemmli buffer • Protein quantification in buffers containing up to 10% SDS • Protein quantification in buffers containing reducing agents (e.g., β-mercaptoethanol (715 mM),    TCEP (50 mM), or DTT) • Protein quantification in buffers containing bromophenol blue (e.g., 0.02%)