Protino GST/4B Columns 1 ml

Protino^® GST/4B Columns 1 ml

Purification of GST-tag proteins

save money without making compromises

Features

Protino GST/4B Columns 1 ml, the cost-effective alternative for FPLC™-based purification of GST-tag proteins

• Highest performance equivalent to GSTrap™ 4B 1 ml columns ® converting is simple
• Simply copy and paste your procedure, no optimization or protocol change necessary
• Suitable for small proteins, large protein complexes, proteins with low expression rates
® universal use
• Huge price advantage against market leader ® great performance for an unbeatable price

Technology
Affinity chromatography

Ligand Glutathione, linked via sulfur atom

Spacer arm
12 atoms

Format
Ready-to-use 1 ml FPLC™ columns

Column volume 1 ml

Column dimensions 0.7 cm (inner diameter) x 2.5 cm (height)

Column body material Polypropylene

Column inlet port 10-32 (1/16'') female

Column outlet port 10-32 (1/16'') male

Matrix

4% beaded agarose

Bead size 90 µm

Binding capacity*

˜10 mg recombinant GST/ml medium

Recommended flow rates

Sample loading**: 0.2 - 1.0 ml/min

Washing/elution: 1.0 ml/min

Chemical stability
Protino GST/4B Columns 1 ml withstand incubation in 0.1 M acetate pH 4,
0.1 M NaOH, 70% ethanol, or 6 M guanidine hydrochloride for 2 hours at room temperature without significant loss of protein yield.

Storage temperature 4-8°C

Storage solution 20% ethanol

Applications

• FPLC™-based purification of GST-tagged proteins
• Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.),
peristaltic pump, or syringe***

* Binding capacity will vary for each GST-tagged protein
** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein
*** Adaptors may be necessary, please contact Technical Service