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Protino® GST/4B Columns

Purification of GST-tag proteins

Leading performance, unbeatable price

Features 


Protino® Glutathione Agarose 4B, the cost-effective alternative for GST-tag protein purification

• Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns
• Simply replace your current products, no optimization or protocol change necessary
• Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use
• Market-leading performance for an unbeatable price

Protino® GST/4B Columns
• Ready-to-use FPLC™ columns filled with Protino® Glutathione Agarose 4B



Protino® GST/4B 1 mL

Protino® GST/4B 5 mL

Technology

Affinity chromatography

Chelating ligand

Glutathione, linked via sulfur atom

Format

1 mL FPLC™ columns

5 mL FPLC™ columns

Column dimensions 0.7 cm (inner diameter) x 2.5 cm (height) 1.6 cm (inner diameter) x 2.5 cm (height)
Column body material

Polypropylen

Polypropylen

Column inlet port

10-32 (1/16'') female

10-32 (1/16'') female

Column outlet port

10-32 (1/16'') male

10-32 (1/16'') male



Matrix

4 % beaded agarose

Bead size

90 µm

90 µm

Binding capacity*

 10 mg

50 mg 

Max. linear flow rate

250 cm/h

 250 cm/h

Max. recommended flow rate

4 mL/min

10 mL/min

Chemical stability

Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate
pH 4, 0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield

Storage temperature

4–8 °C

4–8 °C

* Binding capacity will vary for each GST-tagged protein


Applications


• Purification of GST-tagged proteins
• Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.),
   peristaltic pump, or syringe (adaptors may be necessary, please contact Technical Service)


 

  
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Ordering information

Product

Pack of

Specification REF
Protino GST/4B Columns 1 mL

5

FPLCTM columns 745510.5
Protino GST/4B Columns 5 mL

1

FPLCTM columns 745515.1

5

as above 745515.5

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