Protino® GST/4B Columns	Purification of GST-tag proteins
Leading performance, unbeatable price
Features
Protino® Glutathione Agarose 4B, the cost-effective alternative for GST-tag protein purification
• Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns • Simply replace your current products, no optimization or protocol change necessary • Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use • Market-leading performance for an unbeatable price Protino® GST/4B Columns • Ready-to-use FPLC™ columns filled with Protino® Glutathione Agarose 4B

	Protino® GST/4B 1 mL	Protino® GST/4B 5 mL
Technology	Affinity chromatography
Chelating ligand	Glutathione, linked via sulfur atom
Format	1 mL FPLC™ columns	5 mL FPLC™ columns
Column dimensions	0.7 cm (inner diameter) x 2.5 cm (height)	1.6 cm (inner diameter) x 2.5 cm (height)
Column body material	Polypropylen	Polypropylen
Column inlet port	10-32 (1/16'') female	10-32 (1/16'') female
Column outlet port	10-32 (1/16'') male	10-32 (1/16'') male
Matrix	4 % beaded agarose
Bead size	90 µm	90 µm
Binding capacity*	10 mg	50 mg
Max. linear flow rate	250 cm/h	250 cm/h
Max. recommended flow rate	4 mL/min	10 mL/min
Chemical stability	Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4, 0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield
Storage temperature	4–8 °C	4–8 °C
* Binding capacity will vary for each GST-tagged protein
Applications
• Purification of GST-tagged proteins • Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.),    peristaltic pump, or syringe (adaptors may be necessary, please contact Technical Service)