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Equipment for NucleoSpin®, NucleoFast®,
and NucleoMag® kits |
High-throughput
applications
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| NucleoSpin® kits |
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NucleoSpin® kits based on silica-membrane technology are available in flexible 8-well strip format for varying throughput and in convenient 96-well format for high-throughput nucleic acid purification.
NucleoSpin® 8 / 96 kits can be processed by centrifugation or under vacuum. The unique MN Wash Plate prevents the bottom of the NucleoSpin® binding strips / plate from being contaminated by ethanolic wash buffers during the washing steps, thus improving the drying of the strips / plate under vacuum and preventing ethanol contamination of the eluted DNA. The risk of cross-contamination is minimized by reduced spraying during filtration and elution steps due to optimized outlets of the NucleoSpin® 8-well strips and NucleoSpin® 96-well plates.
Required hardware
Centrifugation:
For centrifugation, a microtiterplate centrifuge is required, which is able to accommodate NucleoSpin® strips / plate stacked on a Round-well Block, Square-well Block, or Rack of Tube Strips (bucket height: 85 mm) and reaches accelerations of 5,600–6,000 x g. For processing NucleoSpin® 8-well strips by centrifugation Starter Set C containing Column Holders C, MN Square-well Blocks, Rack of Tube Strips is additionally required.
Vacuum processing:
A NucleoVac 96 vacuum manifold is required for processing under vacuum (click here for details). For processing NucleoSpin 8-well strips under vacuum Starter Set A containing Column Holders A and NucleoSpin® Dummy Strips is required, too.
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| NucleoFast® kits |
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NucleoFast® kits can be processed by centrifugation or under vacuum. For centrifugation, a microtiterplate centrifuge is required, which is able to accommodate NucleoFast® Plates stacked on a suitable waste collection plate, for example, MN Square-well Block, and reaches accelerations of 4,500 x g. For vacuum processing, the NucleoVac 96 Vacuum Manifold or other suitable vacuum manifold is required (click here for details).
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| NucleoMag® kits |
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For manual processing of NucleoMag® kits a suitable magnetic separator is required, for example, NucleoMag® SEP (click here for details).
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