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Equipment for NucleoSpin, NucleoFast,
and NucleoMag kits

High-Throughput
Applications

NucleoSpin kits

NucleoSpin kits based on silica-membrane technology are available in flexible 8-well strip format for varying throughput and in convenient 96-well format for high-throughput nucleic acid purification.

NucleoSpin 8/96 kits can be processed by centrifugation or under vacuum. The unique MN Wash Plate prevents the bottom of the NucleoSpin binding strips/plate from being contaminated by ethanolic wash buffers during the washing steps, thus improving the drying of the strips/plate under vacuum and preventing ethanol contamination of the eluted DNA. The risk of cross-contamination is minimized by reduced spraying during filtration and elution steps due to optimized outlets of the NucleoSpin 8-well strips and NucleoSpin 96-well plates.

Required hardware

Centrifugation:
For centrifugation, a microtiterplate centrifuge* is required, which is able to accommodate NucleoSpin strips / plate stacked on a Round-well Block, Square-well Block, or Rack with Tube Strips (bucket height: 85 mm) and reaches accelerations of 5 600 – 6 000 x g. For processing NucleoSpin 8-well strips by centrifugation Starter Set C containing Column Holders C, MN Square-well Blocks, Rack with Tube Strips is additionally required.

Vacuum processing:
A NucleoVac 96 vacuum manifold is required for processing under vacuum (click here for details). For processing NucleoSpin 8-well strips under vacuum Starter Set A containing Column Holders A and Dummy Strips is required, too.

 

NucleoFast kits

NucleoFast kits can be processed by centrifugation or under vacuum. For centrifugation, a microtiterplate centrifuge* is required, which is able to accommodate NucleoFast Plates stacked on a suitable waste collection plate, e. g. MN Square-well Block, and reaches accelerations of 4 500 x g. For vacuum processing the NucleoVac 96 Vacuum Manifold or other suitable vacuum manifold is required (click here for details).

 

NucleoMag kits

For manual processing of NucleoMag kits a suitable magnetic separator is required, e. g. NucleoMag SEP (click here for details).

 

*e. g. Hermle Z 513 / Z 513 K, Jouan KR4i, Heraeus Kendro Multifuge® 3 / 3-R, Beckman Coulter Allegra™ 25R, 
Hettich Rotanta 460 series, Sigma 4-15 / 4K15 / 6-15 / 6K15, Eppendorf 5804 / 5804R / 5810 / 5810R (for selected applications only as the maximal g-force is 2130 x g (rotor A-2-DWP) or 2916 x g (rotor A-4-81-MTP/Flex), for detailed information please contact Technical Service Bioanalysis).

  
     
  
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