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NucleoSpin® 8/96 Blood

Application notes
Application Notes NucleoSpin® 8/96 Blood

  
     

NucleoSpin® 8 / 96 Blood
NucleoSpin® 8 / 96 Blood Core Kit

Genomic DNA from blood and biological fluids


Features 

Isolation of genomic DNA from whole blood in flexible 8-well strip format and for high throughput in convenient 96-well format

• Time-saving parallel isolation of genomic DNA from whole blood
• Optimized for manual or automated use under vacuum
• Improved flow-rate reduces risk of clogging no cross-contamination,
   reliable results
• Innovative MN Wash Plate minimizes risk of cross-contamination
• Complete processing possible at room temperature
• Consistently high yields
• NucleoSpin® 8 / 96 Blood Core Kit: Kits with basic content focussed on automation platforms.
   Additional accessories can be combined as needed, click here for details

 


NucleoSpin® 8 Blood
NucleoSpin® 8 Blood Core Kit

NucleoSpin® 96 Blood
NucleoSpin® 96 Blood Core Kit

Technology

Silica-membrane technology

Format

8-well strips

96-well plates

Processing

Manual or automated, optimized for vacuum processing

Sample material

Whole blood treated with EDTA, citrate, heparin, CPDA, human or animal blood
200 μL whole blood, 2 x 106 cultured cells

Fragment size

300 bp–approx. 50 kbp

300 bp–approx. 50 kbp

Typical yield

4–6 µg

4–6 µg

A260/A280

1.8–1.9

1.8–1.9

Elution volume

100 µL

100 µL

Preparation time

35 min/6 strips

70 min/plate

Binding capacity

20 µg

20 µg


Applications*

• Manual or automated isolation of genomic DNA from whole blood or cultured cells
• Typical downstream applications: PCR, Southern blotting, any kind of enzymatic reaction


* Kits to be used for research purposes only!

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® 8 Blood

12 x 8

NucleoSpin® Blood Binding Strips, MN Wash Plates, Rack of Tube Strips, buffers, Proteinase K

740664

60 x 8

as above 740664.5
NucleoSpin® 8 Blood Core Kit

48 x 8

NucleoSpin® Blood Binding Strips, buffers, Proteinase K

740455.4
NucleoSpin® 96 Blood

1 x 96

NucleoSpin® Blood Binding Plate, MN Wash Plate, Lysis Block, Rack of Tube Strips, Tubes (15 mL), buffers, Proteinase K

740665.1

4 x 96

as above 740665.4

24 x 96

as above 740665.24
NucleoSpin® 96 Blood Core Kit

4 x 96

NucleoSpin® Blood Binding Plates, buffers, 
Proteinase K

740456.4
Product accessories

Pack of

Specification REF
NucleoVac 96 Vacuum Manifold

1

740681
NucleoVac Vacuum Regulator

1

for controlling of vacuum 740641
Starter Set A

1

for processing NucleoSpin® 8-well strips under vacuum on NucleoVac 96 Vacuum Manifold 740682
Round-well Block with Cap Strips

4 sets

1 set consists of 1 Round-well Block and 12 Cap Strips 740475

24 sets

as above 740475.24
Rack of Tube Strips

4 sets

1 set consists of 1 rack, 12 strips with 8 tubes each, and 12 Cap Strips 740477

24 sets

as above 740477.24
Cap Strips

48

for sealing of Tube Strips, Round-well Blocks 740478

288

as above 740478.24
MN Square-well Block

4

96-well blocks with 2.1 mL square wells 740476

24

as above 740476.24
Square-well Block

4

740481

24

740481.24
MN Wash Plate

4

to facilitate drying of the DNA/RNA binding module and to minimize the risk of cross-contamination 740479

24

as above 740479.24
Elution Plate U-bottom

24

96-well microplates with 300 µL U-bottom wells, with Self-adhering Foil  740486.24
Self-adhering PE-Foil

50

adhesive tape pads for sealing 96-well plates and 96-well blocks 740676

For ordering click here

  
     
  
     
  
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