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NucleoSpin® 8/96 Plasmid
NucleoSpin® 8/96 Plasmid Core Kit |
Sequencing-grade plasmid DNA
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Features
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Isolation of plasmid DNA in flexible 8-well strip format and for high throughput
in convenient 96-well plate format |
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• Time-saving parallel isolation of plasmid DNA
• Processing of up to 5 mL bacterial culture
• NucleoSpin® 8 Filter Strips/96 Filter Plate for convenient filtration of bacterial lysates
• Processing possible under vacuum or by centrifugation
• Suitable for manual or automated processing under vacuum
• Innovative MN Wash Plate minimizes risk of cross-contamination
• NucleoSpin® 8/96 Plasmid Core Kits: Kits with basic content focussed on automation platforms.* Additional accessories can be combined as needed.
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NucleoSpin® 8 Plasmid
NucleoSpin® 8 Plasmid Core Kit
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NucleoSpin® 96 Plasmid
NucleoSpin® 96 Plasmid Core Kit
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| Technology |
Silica-membrane technology
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| Format |
8-well strips
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96-well plates
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| Processing |
Manual and automated,
vacuum
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Manual and automated,
vacuum or centrifugation
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| Lysate clarification |
8-well filter strips
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96-well filter plates
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| Sample material |
1–5 mL E. coli culture
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1–5 mL E. coli culture
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| Vector size |
< 15 kbp
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< 15 kbp
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| Typical yield |
4–6 µg/mL E. coli culture
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4–6 µg/mL E. coli culture
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| A260/A280 |
1.70–1.85
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1.70–1.85
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| Elution volume |
75–150 µL
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75–150 µL
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| Preparation time |
45 min/6 strips
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45 min/plate
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| Binding capacity |
20 µg
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20 µg
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| Applications |
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• Manual and automated isolation of high-copy plasmids
• Support protocol available for purification of cosmids
• Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis
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| * The NucleoSpin® 8/96 Plasmid Core Kits provide the buffers, RNase A, NucleoSpin® Plasmid Filter Strips/Plate, and NucleoSpin® Plasmid Binding Strips/Plate only. Accessory components (e.g., culture plate, elution plate, MN Wash Plate, and Wash Buffer AW) are not provided in the core kits but can individually be selected from a variety of suitable accessories (see ordering information). This allows highest flexibility for the user. |
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Ordering information
| Product |
Preps
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Specification |
REF |
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NucleoSpin® 8 Plasmid
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12 x 8
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NucleoSpin® Plasmid Filter Strips, NucleoSpin® Plasmid Binding Strips, Culture Plate, MN Wash Plate, Rack of Tube Strips, buffers, RNase A |
740621 |
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60 x 8
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as above |
740621.5 |
| NucleoSpin® 8 Plasmid Core Kit |
48 x 8
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NucleoSpin® Plasmid Filter Strips, NucleoSpin® Plasmid Binding Strips, buffers, RNase A |
740461.4 |
| NucleoSpin® 96 Plasmid |
1 x 96
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NucleoSpin® Plasmid Filter Plate, NucleoSpin® Plasmid Binding Plate, MN Wash Plate, MN Square-well Block, Elution Plate U-bottom, Gas-permeable Foil, Self-adhering Foil, buffers, RNase A
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740625.1 |
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4 x 96
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as above |
740625.4 |
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24 x 96
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as above |
740625.24 |
| NucleoSpin® 96 Plasmid Core Kit |
4 x 96
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NucleoSpin® Plasmid Filter Plates, NucleoSpin® Plasmid Binding Plates, buffers, RNase A
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740616.4 |
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| Product accessories |
Pack of
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Specification |
REF |
| NucleoVac 96 Vacuum Manifold |
1
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740681 |
| NucleoVac Vacuum Regulator |
1
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for controlling of vacuum |
740641 |
| Starter Set A |
1
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for use of NucleoSpin® 8-well strips on the NucleoVac 96 Vacuum Manifold |
740682 |
| Culture Plate |
4 sets
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Square-well Blocks with Gas-permeable Foil |
740488 |
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24 sets
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as above |
740488.24 |
| MN Square-well Block |
4
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96-well blocks with 2.1 mL square wells |
740476 |
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24
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as above |
740476.24 |
| Round-well Block with Cap Strips |
4 sets
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1 set consists of 1 Round-well Block and 12 Cap Strips |
740475 |
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24 sets
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as above |
740475.24 |
| Rack of Tube Strips |
4 sets
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1 set consists of 1 rack, 12 strips with 8 tubes each, and 12 Cap Strips |
740477 |
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24 sets
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as above |
740477.24 |
| Round-well Block Low |
4 sets
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96-well blocks with 0.8 mL round wells and Self-adhering Foil |
740487 |
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24 sets
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as above |
740487.24 |
| Buffer AW |
1 L
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wash buffer |
740916.1 |
For ordering click here
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