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Instruction leaflet:
NUCLEODUR®
NH2 / NH2-RP

  
     
NUCLEODUR® NH2 / NH2-RP
High purity silica phase for HPLC
Aminopropyl modification for NP and RP separations
2.5 % C · not endcapped · USP L8
Properties
Key features:
Multi-mode columns (for RP, NP and IC)
Stable against hydrolysis at low pH (working range pH 2–8), 100 % stable in water;
suitable for LC/MS
Widens scope of analytical HPLC into the polar range
Technical characteristics:
Aminopropyl modified high purity silica; pore size 110 Å, particles sizes 3, 5 and 7 µm;
2.5 % C; not endcapped
Recommended application:
polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditions
For applications click here.
 
Normal phase chromatography (NP) with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
Reversed phase chromatography (RP) of polar compounds in aqueous-organic eluent systems
Ion exchange chromatography of anions and organic acids using conventional buffers and organic modifiers


EC standard columns for analytical HPLC
Ordering information
Length ->   100 mm 125 mm 150 mm 250 mm
NUCLEODUR® 100-3 NH2-RP (3 µm particle size, eluent in column acetonitrile – water)
2 mm ID   760740.20 760741.20    
4.6 mm ID       760742.46 760739.46
EC guard columns* 4 x 2 mm 761951.20   4 x 3 mm 761951.30
CC guard columns** 8 x 3 mm 761035.30   8 x 4 mm 761035.40
NUCLEODUR® 100-5 NH2-RP (5 µm particle size, eluent in column acetonitrile – water)
2 mm ID     760730.20   760732.20
3 mm ID     760730.30   760732.30
4 mm ID     760730.40   760732.40
4.6 mm ID     760730.46 760731.46 760732.46
EC guard columns* 4 x 2 mm 761953.20   4 x 3 mm 761953.30
CC guard columns** 8 x 3 mm 761137.30   8 x 4 mm 761137.40
NUCLEODUR® 100-5 NH2 (5 µm particle size, eluent in column n-heptane)
4 mm ID     760720.40   760722.40
4.6 mm ID     760720.46 760721.46 760722.46
EC guard columns*

  4 x 3 mm 761952.30
CC guard columns**       8 x 4 mm 761130.40
Guard collumns in packs of 3, columns in packs of 1.

Guard columns for EC columns   EC columns with ID Use guard
column holder
      2 mm 3 mm 4 mm 4.6 mm
* Column Protection System EC guard column 4/2 4/3 4/3 4/3 REF 718966
** ChromCart® guard column system CC guard column 8/3 8/3 8/4 8/4 REF 721359
Custom-packed EC columns with other dimensions are available on request.

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