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NUCLEODUR® NH2 / NH2-RP
high purity silica phase for HPLC
aminopropyl modification for NP and RP separations
2.5 % C · not endcapped · USP L8
Features

key features:
multi-mode columns (for RP, NP and IC)
stable against hydrolysis at low pH (working range pH 2 – 8), 100 % stable in water; suitable for LC/MS
widens scope of analytical HPLC into the polar range
technical characteristics:
aminopropyl-modified high purity silica; pore size 110 Å, particles sizes 3, 5 and 7 µm; 2.5 % C; not endcapped
recommended application:
polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditions
 
normal phase chromatography (NP) with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
reversed phase chromatography (RP) of polar compounds in aqueous-organic eluent systems
ion exchange chromatography of anions and organic acids using conventional buffers and organic modifiers


EC standard columns for analytical HPLC
Ordering information

Length ->   100 mm 125 mm 150 mm 250 mm  
NUCLEODUR® 100-3 NH2-RP, 3 µm particle size (eluent in column acetonitrile / water)
2 mm ID   760740.20 760741.20      
4.6 mm ID       760742.46 760739.46  
Guard columns *: 8 x 3 mm 761035.30   8 x 4 mm 761035.40  
NUCLEODUR® 100-5 NH2-RP, 5 µm particle size (eluent in column acetonitrile / water)
2 mm ID     760730.20   760732.20  
3 mm ID
    760730.30   760732.30  
4 mm ID     760730.40   760732.40  
4.6 mm ID     760730.46 760731.46 760732.46  
Guard columns *: 8 x 3 mm 761137.30   8 x 4 mm 761137.40  
NUCLEODUR® 100-5 NH2, 5 µm particle size (eluent in column n-heptane)
4 mm ID     760720.40   760722.40  
4.6 mm ID     760720.46 760721.46 760722.46  
Guard columns *:       8 x 4 mm 761130.40  
Guard collumns in packs of 3, columns in packs of 1.
* Guard column cartridges are coupled to EC columns using guard column adaptor EC (REF 721359). 3 mm ID ChromCart® (CC) guard column cartridges (.30) are used for 2 mm ID and 3 mm ID EC columns; 4 mm ID (.40) CC guard column cartridges are applied for 4 and 4.6 mm ID EC columns.
Custom-packed EC columns with oder dimensions are available on request.

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