Protino Glutathione Agarose 4B

Protino^® Glutathione Agarose 4B

Purification of GST-tag proteins

Leading performance, unbeatable price

Features

Protino® Glutathione Agarose 4B, the cost-effective alternative for GST-tag protein purification

• Simply replace your current products, no optimization or protocol change necessary
• Suitable for small proteins, large protein complexes, proteins with low expression rates
→ universal use
• Market-leading performance for an unbeatable price

Protino® Glutathione Agarose 4B
• Aqueous suspension
• For batch binding, gravity flow, and FPLC™ → highest flexibility of applications
• Empty Protino® columns for gravity flow available

Technology
Affinity chromatography

Chelating ligand Glutathione, linked via sulfur atom

Format
75 % (v/v) aqueous suspension containing 20 % ethanol

Matrix

4 % beaded agarose

Bead size 90 µm

Binding capacity*

> 8 mg/mL

Recommended flow rates

1 mL bed volume (column with 6.6 mm inner diameter):

Sample loading**: 0.2–1.0 mL/min

Washing / elution: 1.0 mL/min

10 mL bed volume (column with 16 mm inner diameter):

Sample loading**: 0.5–5.0 mL/min

Washing / elution: 5.0 mL/min

Max. linear flow rate 250 cm/h

Chemical stability
Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4,
0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield

Storage temperature 4–8 °C

Note Do not autoclave the gel

* Binding capacity will vary for each GST-tagged protein
** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein

Applications

• Purification of GST-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications