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Protino® technology and products
Features
Protino® Ni-IDA
Purification of polyhistidine (His)-tagged
proteins
Protino® Ni-TED
Purification of polyhistidine (His)-tagged
proteins
Technology Affinity chromatography
(IMAC, immobilized metal ion affinity chromatography)
Affinity chromatography
(IMAC, immobilized metal ion affinity chromatography)
Material / backbone Macroporous silica with immobilized Ni2+ Macroporous silica with immobilized Ni2+
Format Dry material Dry material
Dry bulk resin, for gravity-flow chromatography, batch binding, MPLC (e.g., FPLCTM) Dry bulk resin, for gravity-flow chromatography, batch binding, MPLC (e.g., FPLCTM)

Gravity-flow columns filled with dry Ni-IDA

Gravity-flow columns filled with dry Ni-TED
96-well plates filled with dry Ni-IDA
Procedure

Principle: interaction between the His-tag
of the recombinant protein and immobilized Ni2+ ions

• Elution with imidazole (structure analogon 
   of histdine, replacement reaction)

Principle: interaction between the His-tag of the recombinant protein and immobilized Ni2+ ions

• Elution with imidazole (structure analogon 
   of histdine, replacement reaction)
Features Dry material, fast and easy handling,
storage at room temperature

Protino® Ni-IDA
IDA (iminodiacetic acid) as chelating ligand
Three binding sites for the His-tag (—>)


High protein yield and high purity

Dry material, fast and easy handling,
storage at room temperature

Protino® Ni-TED
TED (tris(carboxymethyl) ethylendiamin) as chelating ligand
One binding site for the His-tag (>)

Highest purity of isolated proteins

 

           

 

Features (continued)
Protino® Ni-NTA
Purification of polyhistidine (His)-tagged
proteins
Protino® Glutathione Agarose 4B
Purificatin of Glutathione-S-transferase (GST)-tagged proteins
Technology Affinity chromatography
(IMAC, Immobilized metal ion affinity chromatography)
Affinity chromatography
Material / backbone 6 % beaded agarose (cross-linked), precharged with Ni2+ 4 % beaded agarose with immobilized glutathione
Bead size 45-165 µm 90 µm
Form 50 % aqueous suspension containing
30 % ethanol
75 % aqueous suspension containing
20 % ethanol

Bulk resin, for gravity-flow chromatography, batch binding, MPLC (e.g., FPLC™)

Bulk resin, for gravity-flow chromatography, batch binding, MPLC (e.g., FPLC™)

FPLC™ columns filled with Ni-NTA agarose FPLC™ columns filled with Glutathione Agarose 4B
Procedure Principle: interaction between the
His-tag of the recombinant protein
and immobilized Ni2+ ions

• Elution with imidazole (structure 
   analogon of histdine, replacement
   reaction)
Principle: interaction between the
GST-tag of the recombinant protein
and immobilized glutathione

• Elution with free glutathion (substrate
   of Glutathione-S-transferase)
Features High binding affinity and high capacity
Ready-to-use and cost-saving





Protino® Ni-NTA
NTA (nitrilotriacetic acid) as chelating ligand
Two binding sites for the His-tag (—>)
Highest protein yield and high purity

Highest performance and cost-saving,
equivalent to Glutathione Sepharose™ 4B

Suitable for small proteins, large protein complexes, proteins with low expression rates

Protino® Glutathione Agarose 4B

            
  
     
  
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