Protino® technology and products

Features

	Protino® Ni-IDA Purification of polyhistidine (His)-tagged proteins	Protino® Ni-TED Purification of polyhistidine (His)-tagged proteins
Technology	Affinity chromatography (IMAC, immobilized metal ion affinity chromatography)	Affinity chromatography (IMAC, immobilized metal ion affinity chromatography)
Material / backbone	Macroporous silica with immobilized Ni2+	Macroporous silica with immobilized Ni2+
Format	Dry material	Dry material
	Dry bulk resin, for gravity-flow chromatography, batch binding, MPLC (e.g., FPLCTM)	Dry bulk resin, for gravity-flow chromatography, batch binding, MPLC (e.g., FPLCTM)
	Gravity-flow columns filled with dry Ni-IDA	Gravity-flow columns filled with dry Ni-TED
	96-well plates filled with dry Ni-IDA	–
Procedure	Principle: interaction between the His-tag of the recombinant protein and immobilized Ni2+ ions • Elution with imidazole (structure analogon     of histdine, replacement reaction)	Principle: interaction between the His-tag of the recombinant protein and immobilized Ni2+ ions • Elution with imidazole (structure analogon     of histdine, replacement reaction)
Features	Dry material, fast and easy handling, storage at room temperature Protino® Ni-IDA IDA (iminodiacetic acid) as chelating ligand Three binding sites for the His-tag (—>) High protein yield and high purity	Dry material, fast and easy handling, storage at room temperature Protino® Ni-TED TED (tris(carboxymethyl) ethylendiamin) as chelating ligand One binding site for the His-tag (—>) Highest purity of isolated proteins

Features (continued)
	Protino® Ni-NTA Purification of polyhistidine (His)-tagged proteins	Protino® Glutathione Agarose 4B Purificatin of Glutathione-S-transferase (GST)-tagged proteins
Technology	Affinity chromatography (IMAC, Immobilized metal ion affinity chromatography)	Affinity chromatography
Material / backbone	6 % beaded agarose (cross-linked), precharged with Ni2+	4 % beaded agarose with immobilized glutathione
Bead site	45-165 µm	90 µm
Form	50 % aqueous suspension containing 30 % ethanol	75 % aqueous suspension containing 20 % ethanol
	Bulk resin, for gravity-flow chromatography, batch binding, MPLC (e.g., FPLC™)	Bulk resin, for gravity-flow chromatography, batch binding, MPLC (e.g., FPLC™)
	FPLC™ columns filled with Ni-NTA agarose	FPLC™ columns filled with Glutathione Agarose 4B
Procedure	Principle: interaction between the His-tag of the recombinant protein and immobilized Ni2+ ions • Elution with imidazole (structure     analogon of histdine, replacement    reaction)	Principle: interaction between the GST-tag of the recombinant protein and immobilized glutathione • Elution with free glutathion (substrate    of Glutathione-S-transferase)
Features	High binding affinity and high capacity Ready-to-use and cost-saving   Protino® Ni-NTA NTA (nitrilotriacetic acid) as chelating ligand Two binding sites for the His-tag (—>) Highest protein yield and high purity	Highest performance and cost-saving, equivalent to Glutathione Sepharose™ 4B Suitable for small proteins, large protein complexes, proteins with low expression rates Protino® Glutathione Agarose 4B