Superparamagnetic beads (non-silica)
Principle: binding (high-salt) – washing – elution (low-salt) • Adsorption of DNA / RNA in the presence of chaotropic salts (hydrate shell of DNA / RNA is reversibly removed) • High-salt / ethanolic washing steps to remove contaminants • Low-salt or water elution (hydrate shell is recovered, DNA is released from the beads)
Highly-pure ready-to-use PCR products, genomic DNA, RNA Easily adapted to automated use, e.g. on KingFisher®, KingFisher® mL, KingFisher® 96, and KingFisher® Flex