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NucleoTrap® |
NucleoTrap® mRNA |
Technology /
separation principle |
Silica-matrix technology /
chaotropic salt binding |
Affinity chromatography |
| Material |
Spherical silica beads |
Oligo(dT) latex beads |
| Format |
Silica bead suspension |
Latex bead suspension – NucleoTrap®
Microfilters for bead separation |
| Procedure |
Principle: binding (high-salt) –
washing – elution (low-salt)
• Adsorption of DNA / RNA
in the presence of chaotropic
salts (hydrate shell of DNA/RNA
is reversibly removed)
• High-salt / ethanolic washing
steps to remove contaminants
• Low-salt or water elution
(hydrate shell is recovered,
DNA / RNA is released from the
matrix) |
Principle: binding (high-salt) –
washing – elution (low-salt)
• Adsorption of poly(A) mRNA on
oligo(dT) latex beads under
high-salt conditions
• High-salt washing steps to
remove contaminants
• Elution of poly(A) mRNA with
RNAse-free Water at elevated
temperature |
| Features / result |
Ready-to-use, sequencing and
PCR-grade DNA
Scalability
Cost efficient |
Ready-to-use poly(A) mRNA |